Academic Journal
A polysaccharide F2 from Grifola frondosa depicted an excellent prebiotic effect against hyperglycemia by modulation of gut microbiota associated with SCFAs and BSH
| العنوان: | A polysaccharide F2 from Grifola frondosa depicted an excellent prebiotic effect against hyperglycemia by modulation of gut microbiota associated with SCFAs and BSH |
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| المؤلفون: | Xie, Ting, Huang, Longhua, Zhong, Wei, Yong, Tianqiao, Hu, Huiping, Xie, Yizhen, Xiao, Chun, Wu, Qingping |
| المصدر: | International Journal of Food Science and Technology ; ISSN 0950-5423 1365-2621 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2025 |
| الوصف: | Summary Grifola frondosa is an edible and medicinal fungus with various biological activities. Previously, we reported Gaylussacia frondosa polysaccharide F2 can improve insulin resistance in diabetic rats. In the present study, our aim was to identify its prebiotic function. The structure of polysaccharide F2 was obtained from gel permeation chromatography (GPC), Infrared (IR) spectroscopy and saccharide mapping. Simulated digestion and fecal bacteria fermentation of F2 in vitro was performed to investigate its digestion and fermentation characteristic. Dynamic changes of molecular weight and composition of F2 during digestion and fermentation were monitored by HPLC. The effects of F2 on intestinal microbiota were studied by 16S rDNA sequencing and bioinformatics analysis. The results of simulated digestion in vitro showed that F2 was not digested in saliva, but minor degraded in stomach and intestine. It was worth noting that the content of polysaccharide F2 decreased and short-chain fatty acids (SCFAs) content increased during the fermentation process with fecal. 16S rDNA sequencing results showed that F2 may reshape the structure of gut microbiota, and enrich microbial communities of Bacteriodes spp., Phascolarctobacterium spp., Enterococcus spp. and Veillonella spp. Data suggested F2 could be utilized by intestinal bacteria to produce hypoglycemic components, which was a promising prebiotic. The hypoglycemic mechanism of F2 may be associated with enhancement of SCFAs-producing bacteria (Enterococcus spp.) and BSH (Bile salt hydrolase)-producing bacteria (Bacteriodes spp. and Enterococcus spp.), acceleration the bile acids metabolism and SCFAs production. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/ijfood/vvae071 |
| DOI: | 10.1093/ijfood/vvae071/61341018/vvae071.pdf |
| الاتاحة: | https://doi.org/10.1093/ijfood/vvae071 https://academic.oup.com/ijfst/advance-article-pdf/doi/10.1093/ijfood/vvae071/61341018/vvae071.pdf |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.488BF107 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/ijfood/vvae071 |
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