Academic Journal
Correlation of fluorescence evolution for quantitative analysis of labels and sensors
| العنوان: | Correlation of fluorescence evolution for quantitative analysis of labels and sensors |
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| المؤلفون: | Pellissier-Tanon, Agnès, Adelizzi, Beatrice, Le Saux, Thomas, Lemarchand, Annie, Jullien, Ludovic |
| المساهمون: | Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640) (PASTEUR), Département de Chimie - ENS-PSL, École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique Théorique de la Matière Condensée (LPTMC), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), ANR-19-CE11-0005,HIGHLIGHT,Imagerie sensible à la phase de nombreux marqueurs réversiblement photocommutables en lumière modulée(2019), ANR-19-CE29-0003,dISCern,Le croisement intersystème dans les protéines fluorescentes: un nouveau mécanisme pour la microscopie de fluorescence confocale à contraste dynamique(2019) |
| المصدر: | ISSN: 0003-2670 ; Analytica Chimica Acta ; https://hal.sorbonne-universite.fr/hal-03775423 ; Analytica Chimica Acta, 2022, 1225, pp.340180. ⟨10.1016/j.aca.2022.340180⟩. |
| بيانات النشر: | CCSD Elsevier Masson |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | titration, correlation, characteristic times, signal-to-noise ratio, reversibly photoswitchable fluorescent protein, calcium sensor, [CHIM.ANAL]Chemical Sciences/Analytical chemistry |
| الوصف: | International audience ; Titration without separation, e.g. quantification of a target species in living cells, is a challenge of analytical chemistry. We perform the selective detection of a target using the kinetics involved in a photochemical process and develop a correlation method that we illustrate by the titration of a fluorescent photoswitcher and the target of a photoswitching sensor. Correlating an input time series and a well-chosen weighting function associated with a variable characteristic time yields a spectrum of characteristic times. The upper integration limit of the correlation output can be chosen to match the argument of an extremum of the spectrum with a characteristic time of the input time series in order to quantify the target. A similar procedure is followed to optimize the signal-to-noise ratio. Selectivity and signal-tonoise ratio associated with 15 weighting functions are theoretically predicted. The results are applied to the titration of the reversibly photoswitchable fluorescent protein Dronpa-2 and the titration of calcium using a reversibly photoswitchable fluorescent sensor. The performance of the correlation method is favorably compared to the one of other dynamic contrast protocols. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/j.aca.2022.340180 |
| الاتاحة: | https://hal.sorbonne-universite.fr/hal-03775423 https://hal.sorbonne-universite.fr/hal-03775423v1/document https://hal.sorbonne-universite.fr/hal-03775423v1/file/pellissier_revised_clean.pdf https://doi.org/10.1016/j.aca.2022.340180 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.487E1C5C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.aca.2022.340180 |
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