Academic Journal
Optimization and characterization of liposome formulation by mixture design.
| العنوان: | Optimization and characterization of liposome formulation by mixture design. |
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| المؤلفون: | Maherani, Benoush, Arab-Tehrany, Elmira, Kheirolomoon, Azadah, Reshetov, Vadim, Stebe, Marie-José, Linder, Michel |
| المساهمون: | Laboratoire d'Ingénierie des Biomolécules (LIBio), Université de Lorraine (UL), Centre Alexis Vautrin (CAV), Laboratory of Biophysics and Biotechnology Belarusian, Belarusian State University, Centre de Recherche en Automatique de Nancy (CRAN), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Structure et Réactivité des Systèmes Moléculaires Complexes (SRSMC), Institut de Chimie - CNRS Chimie (INC-CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 0003-2654. |
| بيانات النشر: | HAL CCSD Royal Society of Chemistry |
| سنة النشر: | 2012 |
| المجموعة: | Université de Lorraine: HAL |
| مصطلحات موضوعية: | [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics |
| الوصف: | International audience ; This study presents the application of the mixture design technique to develop an optimal liposome formulation by using the different lipids in type and percentage (DOPC, POPC and DPPC) in liposome composition. Ten lipid mixtures were generated by the simplex-centroid design technique and liposomes were prepared by the extrusion method. Liposomes were characterized with respect to size, phase transition temperature, ζ-potential, lamellarity, fluidity and efficiency in loading calcein. The results were then applied to estimate the coefficients of mixture design model and to find the optimal lipid composition with improved entrapment efficiency, size, transition temperature, fluidity and ζ-potential of liposomes. The response optimization of experiments was the liposome formulation with DOPC: 46%, POPC: 12% and DPPC: 42%. The optimal liposome formulation had an average diameter of 127.5 nm, a phase-transition temperature of 11.43 °C, a ζ-potential of -7.24 mV, fluidity (1/P)(TMA-DPH)((¬)) value of 2.87 and an encapsulation efficiency of 20.24%. The experimental results of characterization of optimal liposome formulation were in good agreement with those predicted by the mixture design technique. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/22158519; hal-00703284; https://hal.science/hal-00703284; PUBMED: 22158519 |
| DOI: | 10.1039/C1AN15794A |
| الاتاحة: | https://hal.science/hal-00703284 https://doi.org/10.1039/C1AN15794A |
| رقم الانضمام: | edsbas.47B32523 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1039/C1AN15794A |
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