Academic Journal
Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process
| العنوان: | Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process |
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| المؤلفون: | Petre, B, Contreras, MP, Bozkurt, TO, Schattat, MH, Sklenar, J, Schornack, S, Abd-El-Haliem, A, Castells-Graells, R, Lozano-Duran, R, Dagdas, YF, Menke, FLH, Jones, AME, Vossen, JH, Robatzek, S, Kamoun, S, Win, J |
| المساهمون: | Biotechnology and Biological Sciences Research Council (BBSRC) |
| المصدر: | 1471 ; 1447 |
| بيانات النشر: | American Society of Plant Biologists |
| سنة النشر: | 2021 |
| المجموعة: | Imperial College London: Spiral |
| مصطلحات موضوعية: | Plant Biology & Botany, 0601 Biochemistry and Cell Biology, 0604 Genetics, 0607 Plant Biology |
| جغرافية الموضوع: | England |
| الوصف: | Pathogens modulate plant cell structure and function by secreting effectors into host tissues. Effectors typically function by associating with host molecules and modulating their activities. This study aimed to identify the host processes targeted by the RXLR class of host-translocated effectors of the potato blight pathogen Phytophthora infestans. To this end, we performed an in planta protein-protein interaction screen by transiently expressing P. infestans RXLR effectors in Nicotiana benthamiana leaves followed by co-immunoprecipitation and liquid chromatography tandem mass spectrometry. This screen generated an effector-host protein interactome matrix of 59 P. infestans RXLR effectors x 586 N. benthamiana proteins. Classification of the host interactors into putative functional categories revealed over 35 biological processes possibly targeted by P. infestans. We further characterized the PexRD12/31 family of RXLR-WY effectors, which associate and co-localize with components of the vesicle trafficking machinery. One member of this family, PexRD31, increased the number of FYVE positive vesicles in N. benthamiana cells. FYVE positive vesicles also accumulated in leaf cells near P. infestans hyphae, indicating that the pathogen may enhance endosomal trafficking during infection. This interactome data set will serve as a useful resource for functional studies of P. infestans effectors and of effector-targeted host processes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1040-4651 |
| Relation: | The Plant Cell; http://hdl.handle.net/10044/1/90283; BB/T006102/1 |
| DOI: | 10.1093/plcell/koab069 |
| الاتاحة: | http://hdl.handle.net/10044/1/90283 https://doi.org/10.1093/plcell/koab069 |
| Rights: | ©The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution,and reproduction in any medium, provided the original work is properly cited ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.46D42BDB |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 10404651 |
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| DOI: | 10.1093/plcell/koab069 |