Academic Journal
Gas chromatography analysis of major free mono- and disaccharides in milk: Method assessment, validation, and application to real samples
| العنوان: | Gas chromatography analysis of major free mono- and disaccharides in milk: Method assessment, validation, and application to real samples |
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| المؤلفون: | IDDA, Ilenia, SPANO, Nadia, CIULU, Marco, NURCHI, Valeria Marina, PANZANELLI, Angelo, PILO, Maria Itria, SANNA, Gavino |
| المساهمون: | Idda, Ilenia, Spano, Nadia, Ciulu, Marco, Nurchi, Valeria Marina, Panzanelli, Angelo, Pilo, Maria Itria, Sanna, Gavino |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Disaccharide, Gas chromatography, Lactose, Milk, Monosaccharide, Analytical Chemistry, Filtration and Separation |
| الوصف: | Saccharides are functional constituents of milk. Although d-lactose represents almost the totality of the saccharides in the milk, minor species, like d-glucose, d-galactose, myo-inositol and, as a result of severe thermal treatments, monosaccharides like d-tagatose, are also detectable. Although chromatography has been the main analytical approach used for accomplishing this task, quite surprisingly a validated gas chromatographic method aimed at the simultaneous determination of these compounds is still needed. Hence, our contribution is devoted to fill this gap. After the optimization of clean-up and derivatization (conversion of saccharides in their trimethyl silyl ethers) steps, the adoption of a highly cross-linked silphenylene stationary phase permitted to obtain high resolution and a fast chromatographic run. Validation was accomplished in terms of limit of detection, limit of quantification, linearity, precision, and trueness. The accuracy of the method was successfully tested on a number of partially skimmed milk samples. Excellent limits of detection for all analytes make this method eligible, also with respect to a gas chromatographic/mass spectrometry approach, for routine analysis and quality control in the dairy industries. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000393030100013; volume:39; issue:23; firstpage:4577; lastpage:4584; numberofpages:8; journal:JOURNAL OF SEPARATION SCIENCE; http://hdl.handle.net/11388/174223; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85000786593; http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1615-9314 |
| DOI: | 10.1002/jssc.201600583 |
| DOI: | 10.1002/(ISSN)1615-9314 |
| الاتاحة: | http://hdl.handle.net/11388/174223 https://doi.org/10.1002/jssc.201600583 http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1615-9314 |
| رقم الانضمام: | edsbas.4630BEF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/jssc.201600583 |
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