Academic Journal
Influence of species and processing parameters on recovery and content of brain tissue-derived extracellular vesicles
| العنوان: | Influence of species and processing parameters on recovery and content of brain tissue-derived extracellular vesicles |
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| المؤلفون: | Huang, Yiyao, Cheng, Lesley, Turchinovich, Andrey, Mahairaki, Vasiliki, Troncoso, Juan C, Pletniková, Olga, Haughey, Norman J, Vella, Laura J, Hill, Andrew F, Zheng, Lei, Witwer, Kenneth W |
| بيانات النشر: | Taylor & Francis |
| سنة النشر: | 2020 |
| المجموعة: | Victoria University Melbourne: VU Eprint Repository |
| مصطلحات موضوعية: | 3101 Biochemistry and cell biology, 3209 Neurosciences, Chancellery, extracellular vesicles, brain health, brain tissue, EVs, disease |
| الوصف: | Extracellular vesicles (EVs) are involved in a wide range of physiological and pathological processes by shuttling material out of and between cells. Tissue EVs may thus lend insights into disease mechanisms and also betray disease when released into easily accessed biological fluids. Since brain-derived EVs (bdEVs) and their cargo may serve as biomarkers of neurodegenerative diseases, we evaluated modifications to a published, rigorous protocol for separation of EVs from brain tissue and studied effects of processing variables on quantitative and qualitative outcomes. To this end, size exclusion chromatography (SEC) and sucrose density gradient ultracentrifugation were compared as final separation steps in protocols involving stepped ultracentrifugation. bdEVs were separated from brain tissues of human, macaque, and mouse. Effects of tissue perfusion and a model of post-mortem interval (PMI) before final bdEV separation were probed. MISEV2018-compliant EV characterization was performed, and both small RNA and protein profiling were done. We conclude that the modified, SEC-employing protocol achieves EV separation efficiency roughly similar to a protocol using gradient density ultracentrifugation, while decreasing operator time and, potentially, variability. The protocol appears to yield bdEVs of higher purity for human tissues compared with those of macaque and, especially, mouse, suggesting opportunities for optimization. Where possible, perfusion should be performed in animal models. The interval between death/tissue storage/processing and final bdEV separation can also affect bdEV populations and composition and should thus be recorded for rigorous reporting. Finally, different populations of EVs obtained through the modified method reported herein display characteristic RNA and protein content that hint at biomarker potential. To conclude, this study finds that the automatable and increasingly employed technique of SEC can be applied to tissue EV separation, and also reveals more about the importance of ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | https://vuir.vu.edu.au/45773/; https://doi.org/10.1080/20013078.2020.1785746; https://vuir.vu.edu.au/45773/1/Influence%20of%20species%20and%20processing%20parameters%20on%20recovery%20and%20content%20of%20brain%20tissue-derived%20extracellular%20vesicles.pdf; Huang, Yiyao, Cheng, Lesley orcid:0000-0002-8075-6144 , Turchinovich, Andrey, Mahairaki, Vasiliki, Troncoso, Juan C, Pletniková, Olga, Haughey, Norman J, Vella, Laura J orcid:0000-0002-1869-6291 , Hill, Andrew F orcid:0000-0001-5581-2354 , Zheng, Lei and Witwer, Kenneth W orcid:0000-0003-1664-4233 (2020) Influence of species and processing parameters on recovery and content of brain tissue-derived extracellular vesicles. Journal of Extracellular Vesicles, 9 (1). ISSN 2001-3078 |
| DOI: | 10.1080/20013078.2020.1785746 |
| الاتاحة: | https://vuir.vu.edu.au/45773/ https://onlinelibrary.wiley.com/doi/10.1080/20013078.2020.1785746 https://doi.org/10.1080/20013078.2020.1785746 https://vuir.vu.edu.au/45773/1/Influence%20of%20species%20and%20processing%20parameters%20on%20recovery%20and%20content%20of%20brain%20tissue-derived%20extracellular%20vesicles.pdf |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.46259301 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1080/20013078.2020.1785746 |
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