Academic Journal
Metabolomic characterisation of progression and spontaneous regression of melanoma in the melanoma-bearing Libechov minipig model
| العنوان: | Metabolomic characterisation of progression and spontaneous regression of melanoma in the melanoma-bearing Libechov minipig model |
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| المؤلفون: | Kertys, Martin, Grendar, Marian, Horak, Vratislav, Zidekova, Nela, Kupcova Skalnikova, Helena, Mokry, Juraj, Halasova, Erika, Strnadel, Jan |
| المصدر: | Melanoma Research ; volume 31, issue 2, page 140-151 ; ISSN 0960-8931 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health) |
| سنة النشر: | 2021 |
| الوصف: | Melanoma-bearing Libechov minipig (MeLiM) represents a large animal model for melanoma research. This model shows a high incidence of complete spontaneous regression of melanoma – a phenomenon uncommon in humans. Here, we present the first metabolomic characterisation of the MeLiM model comparing animals with progressing and spontaneously regressing melanomas. Plasma samples of 19 minipigs with progression and 27 minipigs with evidence of regression were analysed by a targeted metabolomic assay based on mass spectrometry detection. Differences in plasma metabolomics patterns were investigated by univariate and multivariate statistical analyses. Overall, 185 metabolites were quantified in each plasma sample. Significantly altered metabolomic profile was found, and 42 features were differentially regulated in plasma. Besides, the machine learning approach was used to create a predictive model utilising Arg/Orn and Arg/ADMA ratios to discriminate minipigs with progressive disease development from minipigs with regression evidence. Our results suggest that progression of melanoma in the MeLiM model is associated with alteration of arginine, glycerophospholipid and acylcarnitines metabolism. Moreover, this study provides targeted metabolomics characterisation of an animal model of melanoma with progression and spontaneous regression of tumours. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1097/cmr.0000000000000722 |
| DOI: | 10.1097/CMR.0000000000000722 |
| الاتاحة: | http://dx.doi.org/10.1097/cmr.0000000000000722 https://journals.lww.com/10.1097/CMR.0000000000000722 |
| رقم الانضمام: | edsbas.45DB8DF3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1097/cmr.0000000000000722 |
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