Academic Journal
In vitro antagonistic activity of Sclerotium sp. by Bacillus sp. ; Actividad antagónica in vitro de Sclerotium sp. por Bacillus sp. ; Atividade antagônica in vitro de Sclerotium sp. por Bacillus sp.
| العنوان: | In vitro antagonistic activity of Sclerotium sp. by Bacillus sp. ; Actividad antagónica in vitro de Sclerotium sp. por Bacillus sp. ; Atividade antagônica in vitro de Sclerotium sp. por Bacillus sp. |
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| المؤلفون: | Zambrano, Melissa Alezandra Romero, Castanharo, Gianny Roger Parra, Aguiar, Raimundo Wagner de Souza, Chagas Junior, Aloisio Freitas |
| المصدر: | Research, Society and Development; Vol. 11 No. 13; e380111335351 ; Research, Society and Development; Vol. 11 Núm. 13; e380111335351 ; Research, Society and Development; v. 11 n. 13; e380111335351 ; 2525-3409 |
| بيانات النشر: | Research, Society and Development |
| سنة النشر: | 2022 |
| المجموعة: | Research, Society and Development (E-Journal) |
| مصطلحات موضوعية: | Biocontrol, Phytopathogenic fungus, Rhizosphere bacteria, Hongo fitopatógeno, Bacterias de la rizosfera, Biocontrole, Fungo fitopatogênico, Bactéria da rizosfera |
| الوصف: | Bacteria from the genus Bacillus sp. have a high potential for the biological control of phytopathogenic fungi. Therefore, in the present research we proposed to evaluate the antagonistic capacity of Bacillus sp. isolates against the phytopathogenic fungus Sclerotium sp. The tests were performed using two methods (dual and modified dual), also using five Bacillus sp. isolates (LBM01, LBM02, LBM03, LBM04 and LBM06). Mycelial growth evaluations were performed on BDA culture medium. All experiments were performed in triplicate and the plates were incubated under a 12 h photoperiod at 28±1 ºC for 7 days. Radial growth of the pathogens was measured at the intervals of 24, 48, 72, 96, 120, 144 and 168 h. The percentage of inhibition was calculated using the equation: I% = [(C-T)/C]x100. All Bacillus sp. isolates tested were able to inhibit the mycelial growth of Sclerotium sp. within 72 h, while isolates LBM01, LBM03 and LBM06 were able to maintain inhibition for 168 h. The isolate LBM06 showed the highest percentage of inhibition in all two culture methods used. ; Las bacterias pertenecientes al género Bacillus sp. tienen un alto potencial para el control biológico de hongos fitopatógenos. Por lo tanto, en la presente investigación nos propusimos evaluar la capacidad antagónica de los aislados de Bacillus sp. contra el hongo fitopatógeno Sclerotium sp. Las pruebas se realizaron utilizando dos métodos (dual y dual modificado), así como cinco aislados de Bacillus sp. (LBM01, LBM02, LBM03, LBM04 y LBM06). Las evaluaciones del crecimiento micelial se realizaron en medio de cultivo BDA. Todos los experimentos se realizaron por triplicado y las placas se incubaron bajo un fotoperiodo de 12 h a 28±1 ºC durante 7 días. El crecimiento radial de los patógenos se midió a intervalos de 24, 48, 72, 96, 120, 144 y 168 horas. El porcentaje de inhibición se calculó mediante la ecuación: I% = [(C-T)/C]x100. Todos los aislados de Bacillus sp. probados fueron capaces de inhibir el crecimiento micelial de Sclerotium sp. en 72 h, ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | Portuguese |
| ردمك: | 978-3-8011-1335-3 3-8011-1335-3 |
| Relation: | https://rsdjournal.org/index.php/rsd/article/view/35351/29821; https://rsdjournal.org/index.php/rsd/article/view/35351 |
| DOI: | 10.33448/rsd-v11i13.35351 |
| الاتاحة: | https://rsdjournal.org/index.php/rsd/article/view/35351 https://doi.org/10.33448/rsd-v11i13.35351 |
| Rights: | Copyright (c) 2022 Melissa Alezandra Romero Zambrano; Gianny Roger Parra Castanharo; Raimundo Wagner de Souza Aguiar; Aloisio Freitas Chagas Junior ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.45C46532 |
| قاعدة البيانات: | BASE |
| ردمك: | 9783801113353 3801113353 |
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| DOI: | 10.33448/rsd-v11i13.35351 |