Academic Journal
Overexpression of {alpha}1-acid glycoprotein in transgenic mice leads to sensitisation to acute colitis
| العنوان: | Overexpression of {alpha}1-acid glycoprotein in transgenic mice leads to sensitisation to acute colitis |
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| المؤلفون: | Hochepied, T, Wullaert, A, Berger, F G, Baumann, H, Brouckaert, P, Steidler, L, Libert, C |
| بيانات النشر: | BMJ Publishing Group Ltd |
| سنة النشر: | 2002 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Inflammation and inflammatory bowel disease |
| الوصف: | Background: α 1 -Acid glycoprotein (α 1 -AGP) is an acute phase protein in most mammalian species whose concentration rises 2–5-fold during an acute phase reaction. Its serum concentration has often been used as a marker of disease, including inflammatory bowel disease (IBD). High α 1 -AGP levels were found to have a prognostic value for an increased risk of relapse in IBD. Aims: To investigate a possible role for increased serum levels of α 1 -AGP in the development of IBD. Methods: Dextran sodium sulphate (DSS) 2% was added to the drinking water of transgenic mice, overexpressing the rat α 1 -AGP gene, to induce acute colitis, thus mimicking the conditions of relapse. Clinical parameters, inflammatory parameters, and histological analyses on colon sections were performed. Results: Homozygous α 1 -AGP-transgenic mice started losing weight and showed rectal bleeding significantly earlier than heterozygous transgenic or wild-type mice. Survival time of homozygous transgenic mice was significantly shorter compared with heterozygous and wild-type mice. The higher susceptibility of homozygous α 1 -AGP-transgenic mice to DSS induced acute colitis was also reflected in higher local myeloperoxidase levels, higher inflammation scores of the colon, and higher systemic levels of interleukin 6 and serum amyloid P component. Local inflammatory parameters were also significantly different in heterozygous transgenic mice compared with wild-type mice, indicating a local dosage effect. In homozygous transgenic mice, significantly higher amounts of bacteria were found in organs but IgA levels were only slightly lower than those of control mice. Conclusion: Sufficiently high serum levels of α 1 -AGP result in a more aggressive development of acute colitis. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://gut.bmj.com/cgi/content/short/51/3/398; http://dx.doi.org/10.1136/gut.51.3.398 |
| DOI: | 10.1136/gut.51.3.398 |
| الاتاحة: | http://gut.bmj.com/cgi/content/short/51/3/398 https://doi.org/10.1136/gut.51.3.398 |
| Rights: | Copyright (C) 2002, BMJ Publishing Group |
| رقم الانضمام: | edsbas.45ACE7D3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1136/gut.51.3.398 |
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