Academic Journal
Vi-deficient and nonfimbriated mutants of Salmonella typhi agglutinate human blood type antigens and are hyperinvasive
| العنوان: | Vi-deficient and nonfimbriated mutants of Salmonella typhi agglutinate human blood type antigens and are hyperinvasive |
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| المؤلفون: | Miyake, Masaki, Zhao, Licheng, Ezaki, Takayuki, Hirose, Kenji, Khan, Abdul Quayum, Kawamura, Yoshiaki, Shima, Ryuichiro, Kamijo, Miki, Masuzawa, Toshiyuki, Yanagihara, Yasutake |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 1998 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Articles |
| الوصف: | We generated nonfimbriated mutants from both Vi-positive and -negative Salmonella typhi to analyze the role of type 1 fimbriae and Vi-antigen in bacterial invasion. A Vi-defective mutant of S. typhi GIFU 10007-3 was more invasive than the wild-type strain GIFU 10007. The wild-type strain expressing Vi-antigen did not agglutinate both Saccharomyces cerevisiae and human erythrocytes but Vi-defective mutants were able to agglutinate S. cerevisiae and human erythrocytes. Nonfimbriated mutants from Vi-negative GIFU 10007-3 lost the ability to adhere to S. cerevisiae but still could agglutinate human erythrocytes. The Vi-negative mutant increased secreted proteins and became 5-fold more invasive than the wild-type strain. Nonfimbriated Vi mutants became 50–120-fold more invasive than the wild-type GIFU 10007. To determine why nonfimbriated Vi mutants still agglutinate human red blood cells, we searched bacterial proteins that could bind human blood-type antigens. We finally identified a candidate 37 kDa outer membrane protein that recognized fucosyl-galactose, a structure common to blood type A, B and H antigens. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://femsle.oxfordjournals.org/cgi/content/short/161/1/75; http://dx.doi.org/10.1111/j.1574-6968.1998.tb12931.x |
| DOI: | 10.1111/j.1574-6968.1998.tb12931.x |
| الاتاحة: | http://femsle.oxfordjournals.org/cgi/content/short/161/1/75 https://doi.org/10.1111/j.1574-6968.1998.tb12931.x |
| Rights: | Copyright (C) 1998, Oxford University Press |
| رقم الانضمام: | edsbas.456EB94 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1574-6968.1998.tb12931.x |
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