Academic Journal
Harmine stimulates proliferation of human neural progenitors
| العنوان: | Harmine stimulates proliferation of human neural progenitors |
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| المؤلفون: | Dakic, Vanja, Maciel, Renata de Moraes, Drummond, Hannah, Nascimento, Juliana M., Trindade, Pablo, Rehen, Stevens K. |
| المساهمون: | National Council for Scientific and Technological Development (CNPq), Foundation for Research Support in the State of Rio de Janeiro (FAPERJ), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Funding Authority for Studies and Projects (FINEP), Brazilian Development Bank (BNDES), São Paulo Research Foundation |
| المصدر: | PeerJ ; volume 4, page e2727 ; ISSN 2167-8359 |
| بيانات النشر: | PeerJ |
| سنة النشر: | 2016 |
| المجموعة: | PeerJ (E-Journal - via CrossRef) |
| الوصف: | Harmine is the β -carboline alkaloid with the highest concentration in the psychotropic plant decoction Ayahuasca. In rodents, classical antidepressants reverse the symptoms of depression by stimulating neuronal proliferation. It has been shown that Ayahuasca presents antidepressant effects in patients with depressive disorder. In the present study, we investigated the effects of harmine in cell cultures containing human neural progenitor cells (hNPCs, 97% nestin-positive) derived from pluripotent stem cells. After 4 days of treatment, the pool of proliferating hNPCs increased by 71.5%. Harmine has been reported as a potent inhibitor of the dual specificity tyrosine-phosphorylation-regulated kinase (DYRK1A), which regulates cell proliferation and brain development. We tested the effect of analogs of harmine, an inhibitor of DYRK1A (INDY), and an irreversible selective inhibitor of monoamine oxidase (MAO) but not DYRK1A (pargyline). INDY but not pargyline induced proliferation of hNPCs similarly to harmine, suggesting that inhibition of DYRK1A is a possible mechanism to explain harmine effects upon the proliferation of hNPCs. Our findings show that harmine enhances proliferation of hNPCs and suggest that inhibition of DYRK1A may explain its effects upon proliferation in vitro and antidepressant effects in vivo . |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.7717/peerj.2727 |
| الاتاحة: | http://dx.doi.org/10.7717/peerj.2727 https://peerj.com/articles/2727.pdf https://peerj.com/articles/2727.xml https://peerj.com/articles/2727.html |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.455F4BC7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.7717/peerj.2727 |
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