Academic Journal
Structure of the outer membrane translocator domain of the Haemophilus influenzae Hia trimeric autotransporter
| العنوان: | Structure of the outer membrane translocator domain of the Haemophilus influenzae Hia trimeric autotransporter |
|---|---|
| المؤلفون: | Guoyu Meng, Neeraj K Surana, Joseph W St, Geme Iii, Gabriel Waksman |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://emboj.embopress.org/content/embojnl/25/11/2297.full.pdf. |
| سنة النشر: | 2006 |
| المجموعة: | CiteSeerX |
| الوصف: | Autotransporter proteins are defined by the ability to drive their own secretion across the bacterial outer membrane. The Hia autotransporter of Haemophilus influenzae belongs to the trimeric autotransporter subfamily and mediates bacterial adhesion to the respiratory epithelium. In this report, we present the crystal structure of the C-terminal end of Hia, corresponding to the entire Hia translocator domain and part of the passenger domain (residues 992-1098). This domain forms a b-barrel with 12 transmembrane b-strands, including four strands from each subunit. The b-barrel has a central channel of 1.8 nm in diameter that is traversed by three N-terminal a-helices, one from each subunit. Mutagenesis studies demonstrate that the transmembrane portion of the three a-helices and the loop region between the a-helices and the neighboring b-strands are essential for stability of the trimeric structure of the translocator domain, and that trimerization of the translocator domain is a prerequisite for translocator activity. Overall, this study provides important insights into the mechanism of translocation in trimeric autotransporters. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1088.5653; http://emboj.embopress.org/content/embojnl/25/11/2297.full.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1088.5653 http://emboj.embopress.org/content/embojnl/25/11/2297.full.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.44FB67BA |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |