Academic Journal
Neutralization sensitivity, fusogenicity, and infectivity of Omicron subvariants
| العنوان: | Neutralization sensitivity, fusogenicity, and infectivity of Omicron subvariants |
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| المؤلفون: | Wang, Xue-Jun, Yao, Lin, Zhang, Hong-Yun, Zhu, Ka-Li, Zhao, Jing, Zhan, Bing-Dong, Li, Yi-Ke, He, Xue-Juan, Huang, Cong, Wang, Zhuang-Ye, Jiang, Ming-Dong, Yang, Peng, Yang, Yang, Wang, Guo-Lin, Wang, Sheng-Qi, Dai, Er-Hei, Gao, Hui-Xia, Ma, Mai-Juan |
| المساهمون: | National Natural Science Foundation of China, Natural Science Foundation of Beijing Municipality, Biological Safety Project |
| المصدر: | Genome Medicine ; volume 14, issue 1 ; ISSN 1756-994X |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2022 |
| الوصف: | Background The emergence of SARS-CoV-2 Omicron subvariants has raised questions regarding resistance to immunity by natural infection or immunization. We examined the sensitivity of Delta and Omicron subvariants (BA.1, BA.1.1, BA.2, BA.2.12.1, BA.4/5, and BA.3) to neutralizing antibodies from BBIBP-CorV-vaccinated and BBIBP-CorV- or ZF2001-boosted individuals, as well as individuals with Delta and BA.1 breakthrough infections, and determined their fusogenicity and infectivity. Methods In this cross-sectional study, serum samples from two doses of BBIBP-CorV-vaccinated individuals 1 ( n = 36), 3 ( n = 36), and 7 ( n = 37) months after the second dose; BBIBP-CorV- ( n = 25) or ZF2001-boosted ( n = 30) individuals; and fully vaccinated individuals with Delta ( n = 30) or BA.1 ( n = 26) infection were collected. The serum-neutralizing reactivity and potency of bebtelovimab were assessed against D614G, Delta, and Omicron subvariants (BA.1, BA.1.1, BA.2, BA.2.12.1, BA.4/5, and BA.3) through a pseudovirus neutralization assay. The fusogenicity and infectivity of D614G, Delta, and Omicron subvariants were determined by cell-cell fusion assay and pseudovirus infection assay, respectively. Results Omicron subvariants markedly escaped vaccine-elicited neutralizing antibodies after two doses of BBIBP-CorV with comparable efficiency. A third dose vaccination of BBIBP-CorV or ZF2001 increased neutralizing antibody titers and breadth against Delta and three Omicron subvariants. Delta and BA.1 breakthrough infections induced comparable neutralizing antibody titers against D614G and Delta variants, whereas BA.1 breakthrough infections elicited a stronger and broader antibody response against three Omicron subvariants than Delta breakthrough infections. BA.2.12.1 and BA.4/5 are more resistant to immunity induced by breakthrough infections. Bebtelovimab had no significant loss of potency against the Delta and Omicron subvariants. Cell culture experiments showed Omicron subvariants to be less fusogenic and have higher ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1186/s13073-022-01151-6 |
| DOI: | 10.1186/s13073-022-01151-6.pdf |
| DOI: | 10.1186/s13073-022-01151-6/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1186/s13073-022-01151-6 https://link.springer.com/content/pdf/10.1186/s13073-022-01151-6.pdf https://link.springer.com/article/10.1186/s13073-022-01151-6/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.444C30E9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s13073-022-01151-6 |
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