Academic Journal
Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: Optimal formulation
| العنوان: | Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: Optimal formulation |
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| المؤلفون: | Coscueta, ER, Sousa, AS, Reis, CA, Pintado, M |
| المساهمون: | Instituto de Investigação e Inovação em Saúde |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2021 |
| المجموعة: | Repositório Aberto da Universidade do Porto |
| مصطلحات موضوعية: | Chitosan / chemistry, Drug Carriers / chemistry, Drug Compounding, Isothiocyanates / chemistry, Microspheres, Olive Oil / chemistry, Solubility, Water / chemistry |
| الوصف: | Phenylethyl isothiocyanate (PEITC), a chemopreventive compound, is highly reactive due to its considerably electrophilic nature. Furthermore, it is hydrophobic and has low stability, bioavailability and bioaccessibility. This restricts its use in biomedical and nutraceutical or food applications. Thus, the encapsulation of this agent has the function of overcoming these limitations, promoting its solubility in water, and stabilizing it, preserving its bioactivity. So, polymeric microparticles were developed using chitosan-olive oil-PEITC systems. For this, an optimisation process (factors: olive oil: chitosan ratio and PEITC: chitosan ratio) was implemented through a 3-level factorial experimental design. The responses were: the particle size, zeta-potential, polydisperse index, and entrapment efficiency. The optimal formulation was further characterised by FTIR and biocompatibility in Caco-2 cells. Optimal conditions were olive oil: chitosan and PEITC: chitosan ratios of 1.46 and 0.25, respectively. These microparticles had a size of 629 nm, a zeta-potential of 32.3 mV, a polydispersity index of 0.329, and entrapment efficiency of 98.49%. We found that the inclusion process affected the optical behaviour of the PEITC, as well as the microparticles themselves and their interaction with the medium. Furthermore, the microparticles did not show cytotoxicity within the therapeutic values of PEITC. Thus, PEITC was microencapsulated with characteristics suitable for potential biomedical, nutraceutical and food applications. ; This research was funded by Foundation for Science and Technology (FCT) and for Competitiveness and Internationalization Operational Program through the project no 032094 GastroCure - Bioactive Soybean and Cruciferous extracts towards application in gastrointestinal disorders: development, characterization and delivery. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | PLoS ONE, vol.16(5):e0248257; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0248257; https://hdl.handle.net/10216/152488 |
| DOI: | 10.1371/journal.pone.0248257 |
| الاتاحة: | https://hdl.handle.net/10216/152488 https://doi.org/10.1371/journal.pone.0248257 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.443FBD92 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0248257 |