التفاصيل البيبلوغرافية
| العنوان: |
Additional file 6 of Ribosomal L1 domain-containing protein 1 coordinates with HDM2 to negatively regulate p53 in human colorectal Cancer cells |
| المؤلفون: |
Li Ding (16240), Zhiping Zhang (279585), Chenhong Zhao (11244627), Lei Chen (54296), Zhiqiang Chen (218692), Jie Zhang (64655), Yaxian Liu (11244630), Yesen Nie (11244633), Yanzhi He (11244636), Kai Liao (636114), Xinyue Zhang (271120) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Cell Biology, Genetics, Molecular Biology, Pharmacology, Biotechnology, Immunology, Developmental Biology, Cancer, Hematology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, RSL1D1, Nucleolar protein, p53, HDM2, mRNA stability, Protein-RNA interaction, Ubiquitination, Protein-protein interaction, Cell proliferation, Cell survival |
| الوصف: |
Additional file 6: Supplementary Fig. S5. Downregulation of HDM2 Does Not Remarkably Change the Expression of RSL1D1 in HCT116p53+/+ Cells. Cells were transfected with siRNA and harvested for determining the mRNA and protein levels of indicated genes 48 h post-transfection. A The mRNA levels of HDM2, RSL1D1, p53, p21, and PUMA were determined by qRT-PCR in siHDM2- or siNC-transfected cells. GAPDH was used as an internal control to normalize the values. The normalized values of siNC-treated cells were set to 1. Data are represented as mean ± SD. Student’s t test. *P < 0.05 and **P < 0.01 denote significant difference. B The levels of HDM2, RSL1D1, p53, p21, and PUMA proteins were evaluated by western blot analysis in siHDM2- or siNC-transfected cells. β-actin was set as a loading control. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Additional_file_6_of_Ribosomal_L1_domain-containing_protein_1_coordinates_with_HDM2_to_negatively_regulate_p53_in_human_colorectal_Cancer_cells/15128580 |
| DOI: |
10.6084/m9.figshare.15128580.v1 |
| الاتاحة: |
https://doi.org/10.6084/m9.figshare.15128580.v1 |
| Rights: |
CC BY + CC0 |
| رقم الانضمام: |
edsbas.44124D28 |
| قاعدة البيانات: |
BASE |