Academic Journal
A purified diet affects intestinal epithelial proliferation and barrier functions through gut microbial alterations
| العنوان: | A purified diet affects intestinal epithelial proliferation and barrier functions through gut microbial alterations |
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| المؤلفون: | Shiratori, Hiroaki, Hattori, Kisara M, Nakata, Kazuaki, Okawa, Takuma, Komiyama, Seiga, Kinashi, Yusuke, Kabumoto, Yuma, Kaneko, Yuria, Nagai, Motoyoshi, Shindo, Tomoko, Moritoki, Nobuko, Kawamura, Yuki I, Dohi, Taeko, Takahashi, Daisuke, Kimura, Shunsuke, Hase, Koji |
| المساهمون: | National Center for Global Health and Medicine, Fuji Foundation for Protein Research |
| المصدر: | International Immunology ; volume 36, issue 5, page 223-240 ; ISSN 1460-2377 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2024 |
| الوصف: | The gut microbiota plays a crucial role in maintaining epithelial barrier function. Although multiple studies have demonstrated the significance of dietary factors on the gut microbiota and mucosal barrier function, the impact of a purified diet, which has long been used in various animal experiments, on intestinal homeostasis remains to be elucidated. Here, we compared the impact of two different types of diets, a crude diet and an AIN-93G-formula purified diet, on epithelial integrity and the gut microbiota. Purified diet-fed mice exhibited shorter villi and crypt lengths and slower epithelial turnover, particularly in the ileum. In addition, antimicrobial products, including REG3γ, were substantially decreased in purified diet-fed mice. Purified diet feeding also suppressed α1,2-fucosylation on the epithelial surface. Furthermore, the purified diet induced metabolic rewiring to fatty acid oxidation and ketogenesis. 16S ribosomal RNA gene sequencing of the ileal contents and mucus layer revealed distinct gut microbiota compositions between the purified and crude diet-fed mice. Purified diet feeding reduced the abundance of segmented filamentous bacteria (SFB), which potently upregulate REG3γ and fucosyltransferase 2 (Fut2) by stimulating group 3 innate lymphoid cells (ILC3s) to produce IL-22. These observations illustrate that the intake of a crude diet secures epithelial barrier function by facilitating SFB colonization, whereas a purified diet insufficiently establishes the epithelial barrier, at least partly owing to the loss of SFB. Our data suggest that the influence of purified diets on the epithelial barrier integrity should be considered in experiments using purified diets. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/intimm/dxae003 |
| DOI: | 10.1093/intimm/dxae003/57066868/dxae003.pdf |
| الاتاحة: | http://dx.doi.org/10.1093/intimm/dxae003 https://academic.oup.com/intimm/advance-article-pdf/doi/10.1093/intimm/dxae003/57066868/dxae003.pdf https://academic.oup.com/intimm/article-pdf/36/5/223/57146381/dxae003.pdf |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.43F65FAB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/intimm/dxae003 |
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