Academic Journal
Model acetylcholinesterase‐Fc fusion glycoprotein biotechnology system for the manufacture of an organophosphorus toxicant bioscavenging countermeasure
| العنوان: | Model acetylcholinesterase‐Fc fusion glycoprotein biotechnology system for the manufacture of an organophosphorus toxicant bioscavenging countermeasure |
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| المؤلفون: | Thomas G. Biel, Talia Faison, Alicia M. Matthews, Uriel Ortega‐Rodriguez, Vincent M. Falkowski, Edward Meek, Xin Bush, Matthew Flores, Sarah Johnson, Wells W. Wu, Mari Lehtimaki, Rong‐Fong Shen, Cyrus Agarabi, V. Ashutosh Rao, Janice E. Chambers, Tongzhong Ju |
| المصدر: | Bioengineering & Translational Medicine, Vol 9, Iss 5, Pp n/a-n/a (2024) |
| بيانات النشر: | Wiley |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | acetylcholinesterase fusion protein, biomanufacturing, bioscavenger, glycosylation, organophosphorus toxicant, Chemical engineering, TP155-156, Biotechnology, TP248.13-248.65, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Organophosphate (OP) toxicants remain an active threat to public health and to warfighters in the military. Current countermeasures require near immediate administration following OP exposure and are reported to have controversial efficacies. Acetylcholinesterase (AChE) fused to the human immunoglobulin 1 (IgG1) Fc domain (AChE‐Fc) is a potential bioscavenger for OP toxicants, but a reproducible AChE‐Fc biomanufacturing strategy remains elusive. This report is the first to establish a comprehensive laboratory‐scale bioprocessing strategy that can reproducibly produce AChE‐Fc and AChE(W86A)‐Fc which is a mutated AChE protein with reduced enzymatic activity. Characterization studies revealed that AChE‐Fc and AChE(W86A)‐Fc are N‐glycosylated dimeric fusion glycoproteins but only AChE‐Fc had the capability to bind to paraoxon (a model OP). This AChE‐Fc fusion glycoprotein bioprocessing strategy can be leveraged during industrial biomanufacturing development, while the research‐grade AChE‐Fc proteins can be used to determine the potential clinical relevance of the countermeasure against OP toxicants. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://doi.org/10.1002/btm2.10666; https://doaj.org/toc/2380-6761; https://doaj.org/article/47e008f8779e454493107a0ed1b19182 |
| DOI: | 10.1002/btm2.10666 |
| الاتاحة: | https://doi.org/10.1002/btm2.10666 https://doaj.org/article/47e008f8779e454493107a0ed1b19182 |
| رقم الانضمام: | edsbas.43724202 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/btm2.10666 |
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