Academic Journal

Regulation of the actin–myosin interaction by titin

التفاصيل البيبلوغرافية
العنوان: Regulation of the actin–myosin interaction by titin
المؤلفون: Niederländer, Nicolas, Raynaud, Fabrice, Astier, Catherine, Chaussepied, Patrick
المصدر: European Journal of Biochemistry ; volume 271, issue 22, page 4572-4581 ; ISSN 0014-2956 1432-1033
بيانات النشر: Wiley
سنة النشر: 2004
المجموعة: Wiley Online Library (Open Access Articles via Crossref)
الوصف: Titin is known to interact with actin thin filaments within the I‐band region of striated muscle sarcomeres. In this study, we have used a titin fragment of 800 kDa (T800) purified from striated skeletal muscle to measure the effect of this interaction on the functional properties of the actin–myosin complex. MALDI‐TOF MS revealed that T800 contains the entire titin PEVK (Pro, Glu, Val, Lys‐rich) domain. In the presence of tropomyosin–troponin, T800 increased the sliding velocity (both average and maximum values) of actin filaments on heavy‐meromyosin (HMM)‐coated surfaces and dramatically decreased the number of stationary filaments. These results were correlated with a 30% reduction in actin‐activated HMM ATPase activity and with an inhibition of HMM binding to actin N‐terminal residues as shown by chemical cross‐linking. At the same time, T800 did not affect the efficiency of the Ca 2+ ‐controlled on/off switch, nor did it alter the overall binding energetics of HMM to actin, as revealed by cosedimentation experiments. These data are consistent with a competitive effect of PEVK domain‐containing T800 on the electrostatic contacts at the actin–HMM interface. They also suggest that titin may participate in the regulation of the active tension generated by the actin–myosin complex.
نوع الوثيقة: article in journal/newspaper
اللغة: English
DOI: 10.1111/j.1432-1033.2004.04429.x
الاتاحة: http://dx.doi.org/10.1111/j.1432-1033.2004.04429.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.2004.04429.x
https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.2004.04429.x
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رقم الانضمام: edsbas.432AECA6
قاعدة البيانات: BASE
الوصف
DOI:10.1111/j.1432-1033.2004.04429.x