Academic Journal
Bioluminescent monitoring of recombinant lactic acid bacteria and their products
| العنوان: | Bioluminescent monitoring of recombinant lactic acid bacteria and their products |
|---|---|
| المؤلفون: | Choi, In Young, Oh, Jee-Hwan, Wang, Zhiying, van Pijkeren, Jan-Peter |
| المساهمون: | Turner, Mark S., National Research Foundation of Korea, U.S. Department of Agriculture, HHS | National Institutes of Health, UW-Madison Food Research Institutes |
| المصدر: | mBio ; volume 14, issue 5 ; ISSN 2150-7511 |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2023 |
| الوصف: | Biotherapeutic strategies to promote health, including the application of engineered microbes to deliver therapeutic molecules, hold strong promise. However, without precision tools to detect therapeutic microbes and their products, we are hampered in our ability to monitor and fine-tune therapeutic delivery. Here, we adapted a bioluminescent peptide tagging system for use in lactic acid bacteria, a group of organisms whose members are commonly exploited as delivery vehicles of therapeutics and vaccines. As a proof of concept, we developed various Limosilactobacillus reuteri strains that each produced a recombinant therapeutic protein with an 11 amino acid tag, which is essential to yield a luminescent signal. Luminescent-based quantification of recombinant protein was more sensitive than commercially available immunoassays. In addition, we demonstrated that the bioluminescent peptide tagging system allows in situ recombinant protein detection in a continuous-culture parallel bioreactor system. This presents an exciting opportunity to determine recombinant protein production dynamics in response to different stimuli. Finally, following oral administration of recombinant microbes, luminescence in intestinal and fecal samples allowed for rapid detection of microbes with equal sensitivity to conventional plate count. Because we demonstrated the functionality of this bioluminescent peptide tagging system in 12 species encompassing nine genera , our approach will create previously unexplored opportunities in lactic acid bacteria research. IMPORTANCE Lactic acid bacteria constitute a genetically diverse group of microorganisms with significant roles in the food industry, biotechnology, agriculture, and medicine. A core understanding of bacterial physiology in diverse environments is crucial to select and develop bacteria for industrial and medical applications. However, there is a lack of versatile tools to track (recombinant) protein production in lactic acid bacteria. In this study, we adapted a ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/mbio.01197-23 |
| الاتاحة: | http://dx.doi.org/10.1128/mbio.01197-23 https://journals.asm.org/doi/pdf/10.1128/mbio.01197-23 |
| Rights: | https://creativecommons.org/licenses/by/4.0/ ; https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.430FCF2C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/mbio.01197-23 |
|---|