Academic Journal
Circulating tumor DNA is a prognostic marker of tumor recurrence in stage II and III colorectal cancer: multicentric, prospective cohort study (ALGECOLS)
| العنوان: | Circulating tumor DNA is a prognostic marker of tumor recurrence in stage II and III colorectal cancer: multicentric, prospective cohort study (ALGECOLS) |
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| المؤلفون: | Benhaim, Léonor, Bouché, Olivier, Normand, Corinne, Didelot, Audrey, Mulot, Claire, Le Corre, Delphine, Garrigou, Sonia, Djadi-Prat, Juliette, Wang-Renault, Shu Fang, Perez-Toralla, Karla, Pekin, Deniz, Poulet, Geoffroy, Landi, Bruno, Taieb, Julien, Selvy, Marie, Emile, Jean François J.F., Lecomte, Thierry, Blons, Heĺène, Chatellier, Gilles, Link, Darren R., Taly, Valérie, Laurent-Puig, Pierre F. |
| المساهمون: | Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École Pratique des Hautes Études (EPHE), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité), Département de chirurgie viscérale Gustave Roussy, Institut Gustave Roussy (IGR), Hôpital universitaire Robert Debré Reims (CHU Reims), Hôpital Européen Georges Pompidou APHP (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Eurofins Biomnis, Service d'oncologie médicale CHU HEGP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou APHP (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), CHU Clermont-Ferrand, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), UFR Sciences de la santé Simone Veil (UVSQ Santé), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay, Hôpital Ambroise Paré AP-HP, CHU Trousseau Tours, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Cancer Research and Personalized Medicine - CARPEM Paris (SIRIC CARPEM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpital Cochin AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Necker - Enfants Malades AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut national du cancer (INCa), This work was supported by the Ministère de l’Enseignement Supérieur et de la Recherche, the Université Paris-Descartes, the Centre National de la Recherche Scientifique (CNRS), the Institut National de la Santé et de la Recherche Médicale (INSERM), the Institut National du Cancer (INCA, n° 2009-1-RT-03-US-1 and 2009-RT-03-UP5-1), the Association pour la recherche contre le cancer (ARC, no. SL220100601375), the Agence Nationale de la Recherche (ANR Nano-biotechnologies, n° ANR-10-NANO-0002-09), the SIRIC CARPEM, the ligue nationale contre le cancer(LNCC, Program ‘Equipe labelisée LIGUE’, no° EL2016.LNCC/VaT), and Advanced Merieux Research Grant (PLP and VT). Dr. L.B. received an INCA fellowship (number 0849). G.P. was supported by a CIFRE funding (number 2018/0368) from ANRT (Association Nationale Recherche Technologie) and Eurofin-Biomnis., ANR-10-NANO-0002,BalTrap,Pinces optiques à base de composants nanophotoniques(2010) |
| المصدر: | ISSN: 0959-8049 ; European Journal of Cancer ; https://hal.science/hal-03524599 ; European Journal of Cancer, 2021, 159, pp.24-33. ⟨10.1016/j.ejca.2021.09.004⟩. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Circulating tumor DNA, Colorectal cancer, Liquid biopsy, Methylated DNA, Picodroplet droplet-based digital PCR, Prognostic marker, [SDV.CAN]Life Sciences [q-bio]/Cancer |
| الوصف: | International audience ; Background: In non-metastatic colorectal cancer (CRC), we evaluated prospectively the pertinence of longitudinal detection and quantification of circulating tumor DNA (ctDNA) as a prognostic marker of recurrence. Method: The presence of ctDNA was assessed from plasma collected before and after surgery for 184 patients classified as stage II or III and at each visit during 3–4 years of follow-up. The ctDNA analysis was performed by droplet-based digital polymerase chain reaction, targeting mutation and methylation markers, blindly from the clinical outcomes. Multivariate analyses were adjusted on age, gender, stage, and adjuvant chemotherapy. Results: Before surgery, 27.5% of patients were positive for ctDNA detection. The rate of recurrence was 32.7% and 11.6% in patients with or without detectable ctDNA respectively (P = 0.001). Time to recurrence (TTR) was significantly shorter in patients with detectable ctDNA before (adjusted hazard ratio [HR] = 3.58, 95% confidence interval [CI] 1.71–7.47) or immediately after surgery (adjusted HR = 3.22, 95% CI 1.32–7.89). The TTR was significantly shorter in patients with detectable ctDNA during the early postoperative follow-up (1–6 months) (adjusted HR = 5, 95% CI 1.9–12.9). Beyond this period, ctDNA remained a prognostic marker with a median anticipated diagnosis of recurrence of 13.1 weeks (interquartile range 28 weeks) when compared to imaging follow-up. The rate of ctDNA+ might be underestimated knowing that consensus pre-analytical conditions were not described at initiation of the study. Conclusion: This prospective study confirms the relevance of ctDNA as a recurrence risk factor in stage II and III CRC before surgery and as a marker of minimal residual disease after surgery that may predict recurrence several months before imaging techniques. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/j.ejca.2021.09.004 |
| الاتاحة: | https://hal.science/hal-03524599 https://hal.science/hal-03524599v1/document https://hal.science/hal-03524599v1/file/PIIS0959804921005980.pdf https://doi.org/10.1016/j.ejca.2021.09.004 |
| Rights: | http://hal.archives-ouvertes.fr/licences/copyright/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.42AF80A4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.ejca.2021.09.004 |
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