التفاصيل البيبلوغرافية
| العنوان: |
Table_1_Astrocyte-derived exosomal miR-378a-5p mitigates cerebral ischemic neuroinflammation by modulating NLRP3-mediated pyroptosis.docx |
| المؤلفون: |
Ruiting Sun, Wenxin Liao, Ting Lang, Keyi Qin, Keyan Jiao, Le Shao, Changqing Deng, Yan She |
| سنة النشر: |
2024 |
| المجموعة: |
Frontiers: Figshare |
| مصطلحات موضوعية: |
Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, astrocyte-derived exosomes, pyroptosis, inflammation, cerebral ischemia, NLRP3, miR-378a-5p |
| الوصف: |
Objective This study aimed to investigate the regulatory role of astrocyte-derived exosomes and their microRNAs (miRNAs) in modulating neuronal pyroptosis during cerebral ischemia. Methods Astrocyte-derived exosomes were studied for treating cerebral ischemia in both in vitro and in vivo models. The effects of astrocyte-derived exosomes on neuroinflammation were investigated by analyzing exosome uptake, nerve damage, and pyroptosis protein expression. High throughput sequencing was used to identify astrocyte-derived exosomal miRNAs linked to pyroptosis, followed by validation via qRT‒PCR. The relationship between these miRNAs and NLRP3 was studied using a dual luciferase reporter assay. This study used miR-378a-5p overexpression and knockdown to manipulate OGD injury in nerve cells. The impact of astrocyte-derived exosomal miR-378a-5p on the regulation of cerebral ischemic neuroinflammation was assessed through analysis of nerve injury and pyroptosis protein expression. Results Our findings demonstrated that astrocyte-derived exosomes were internalized by neurons both in vitro and in vivo. Additionally, Astrocyte-derived exosomes displayed a neuroprotective effect against OGD-induced neuronal injury and brain injury in the ischemic cortical region of middle cerebral artery occlusion (MCAO) rats while also reducing pyroptosis. Further investigations revealed the involvement of astrocyte-derived exosomal miR-378a-5p in regulating pyroptosis by inhibiting NLRP3. The overexpression of miR-378a-5p mitigated neuronal damage, whereas the knockdown of miR-378a-5p increased NLRP3 expression and exacerbated pyroptosis, thus reversing this neuroprotective effect. Conclusion Astrocyte-derived exosomal miR-378a-5p has a neuroprotective effect on cerebral ischemia by suppressing neuroinflammation associated with NLRP3-mediated pyroptosis.Further research is required to comprehensively elucidate the signaling pathways by which astrocyte-derived exosomal miR-378a-5p modulates neuronal pyroptosis. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/Table_1_Astrocyte-derived_exosomal_miR-378a-5p_mitigates_cerebral_ischemic_neuroinflammation_by_modulating_NLRP3-mediated_pyroptosis_docx/26517991 |
| DOI: |
10.3389/fimmu.2024.1454116.s001 |
| الاتاحة: |
https://doi.org/10.3389/fimmu.2024.1454116.s001
https://figshare.com/articles/dataset/Table_1_Astrocyte-derived_exosomal_miR-378a-5p_mitigates_cerebral_ischemic_neuroinflammation_by_modulating_NLRP3-mediated_pyroptosis_docx/26517991 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.428B1FA7 |
| قاعدة البيانات: |
BASE |