التفاصيل البيبلوغرافية
| العنوان: |
Protocol for precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. |
| المؤلفون: |
Nievergelt, A., Diener, D., Bogdanova, A., Brown, T., Pigino, G. |
| المصدر: |
STAR protocols |
| سنة النشر: |
2024 |
| المجموعة: |
Max Planck Society: MPG.PuRe |
| الوصف: |
CRISPR-Cas genome engineering in the unicellular green algal model Chlamydomonas reinhardtii has until recently suffered from low integration efficiencies despite traditional genetics being well established. Here, we present a protocol for efficient homology-directed knockin mutagenesis in all commonly used strains of Chlamydomonas. We describe steps for scarless integration of fusion tags and sequence modifications of almost all proteins without the need for a preceding mutant line. We further empower this genetic-editing approach by efficient crossing and highly robust screening protocols. For complete details on the use and execution of this protocol, please refer to Nievergelt et al. (2023).1. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/38096061; http://hdl.handle.net/21.11116/0000-000E-AB3A-1 |
| الاتاحة: |
http://hdl.handle.net/21.11116/0000-000E-AB3A-1 |
| رقم الانضمام: |
edsbas.4260EB41 |
| قاعدة البيانات: |
BASE |