Academic Journal
Helicobacter hepaticus Does Not Induce or Potentiate Colitis in Interleukin-10-Deficient Mice
| العنوان: | Helicobacter hepaticus Does Not Induce or Potentiate Colitis in Interleukin-10-Deficient Mice |
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| المؤلفون: | Dieleman, L. A., Arends, A., Tonkonogy, S. L., Goerres, M. S., Craft, D. W., Grenther, W., Sellon, R. K., Balish, E., Sartor, R. B. |
| المصدر: | Infection and Immunity, 68(9) |
| سنة النشر: | 2000 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | Experimental disease, Vertebrata, Souris, Mouse, Spirillaceae, Infection, Interleukin 10, Colite, Bacteria, Interleukine 10, Spirillales, Pathologie expérimentale, Mammalia, Intestinal disease, Helicobacter hepaticus, Intestin pathologie, Bactérie, Colitis, Rodentia, Cytokine, Bactériose, Bacteriosis, Digestive diseases |
| الوصف: | Helicobacter hepaticus has been reported to induce colitis, hepatitis, and hepatocellular carcinoma in several different murine models. The aim of this study was to determine if H. hepaticus will cause colitis in monoassociated mice lacking the interleukin-10 gene (IL-10−/− mice) and potentiate colitis in specific-pathogen-free (SPF) IL-10−/− mice. Germfree IL-10−/− mice on either a mixed (C57BL/6 × 129/Ola) or inbred (129/SvEv) genetic background were monoassociated with H. hepaticus ATCC 51448 by oral feeding and rectal enemas. In a second experiment, germfree IL-10−/− mice were colonized with stool from SPF mice that harbored or did not harbor endogenous H. hepaticus. After 7 to 9 weeks of colonization, weight loss and mortality were assessed, the colon was isolated for histology and IL-12 secretion, and mesenteric lymph node cells were assessed for T-cell activation markers. It was found that IL-10−/− mice monoassociated with H. hepaticus for up to 16 weeks showed almost no histologic colitis or increased IL-12 production. SPF IL-10-knockout mice had no significant difference in weight loss, mortality rate, histologic scores, colonic IL-12 secretion, or T-cell activation with or without H. hepaticus. We conclude that H. hepaticus does not induce or potentiate disease in our IL-10−/− mice and therefore is not required to induce colitis in genetically susceptible hosts. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://doi.org/10.17615/8afe-dr98; https://cdr.lib.unc.edu/downloads/qj72pf68m?file=thumbnail; https://cdr.lib.unc.edu/downloads/qj72pf68m |
| DOI: | 10.17615/8afe-dr98 |
| الاتاحة: | https://doi.org/10.17615/8afe-dr98 https://cdr.lib.unc.edu/downloads/qj72pf68m?file=thumbnail https://cdr.lib.unc.edu/downloads/qj72pf68m |
| Rights: | http://rightsstatements.org/vocab/InC/1.0/ |
| رقم الانضمام: | edsbas.41B29B6D |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/8afe-dr98 |
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