Academic Journal
THE MOLECULAR BASIS OF DIABETIC CARDIOMYOPATH
| العنوان: | THE MOLECULAR BASIS OF DIABETIC CARDIOMYOPATH |
|---|---|
| المؤلفون: | Ali Sultan, Ahmed Salaheldin Mustafa |
| المصدر: | Dissertations |
| بيانات النشر: | Scholarworks@UAEU |
| سنة النشر: | 2022 |
| المجموعة: | United Arab Emirates University: Scholarworks@UAEU / جامعة الامارات |
| مصطلحات موضوعية: | Heart, Diabetes, Obesity, Diabetic Cardiomyopathy, Zucker diabetic fatty rat, Zucker fatty rat, Zucker lean rat, Medicine and Health Sciences |
| الوصف: | Diabetes mellitus (DM) is a major and worsening global health problem. Type 2 diabetes mellitus (T2DM) accounts for more than 90% of DM and the global epidemic of obesity largely explains the dramatic increase in the incidence and prevalence of T2DM over the past 20 years. Cardiovascular complications are the major cause of morbidity and mortality in diabetic patients. The electrocardiogram (ECG) of diabetic and obese patients is frequently disturbed. The aim of this project was to characterize and clarify the molecular basis of electro-mechanical dysfunction in the hearts of type 2 diabetic and type 2 diabetic/obese rats. Experiments were performed in Zucker diabetic fatty (ZDF), Zucker fatty (ZF), and Zucker lean (ZL) rats. In vivo, biotelemetry experiments were performed to establish how the ECG was altered by diabetes and diabesity. Experiments were also carried out in isolated hearts to investigate further how diabetes and diabesity affect the electrical conduction system of the heart. Cell imaging was employed to assess ventricular myocyte shortening. Fluorescence photometry and whole-cell patch-clamp techniques were used to assess the effects of diabetes and diabesity on ion channel currents. Molecular biology and electron microscopy techniques were employed to assess proteins and structures associated with cardiac muscle contraction. Heart rate (HR) was reduced by aging and by diabesity in the absence of changes in physical activity and body temperature. Reductions in heart rate variability linked with altered sympathovagal drive may partly underlie disturbed HR in the ZDF rat even in the absence of autonomic nervous system control in the isolated perfused heart. The amplitude of shortening is generally well preserved in ZDF myocytes. There was evidence of altered time course of the Ca2+ transient and shortening in ventricular myocytes from ZDF rats. Molecular and structural defects in the ZF and ZDF rat hearts were observed. The results suggest that sarcoplasmic reticulum (SR) Ca2+ handling as well as ... |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | https://scholarworks.uaeu.ac.ae/all_dissertations/170; https://scholarworks.uaeu.ac.ae/cgi/viewcontent.cgi?article=1169&context=all_dissertations |
| الاتاحة: | https://scholarworks.uaeu.ac.ae/all_dissertations/170 https://scholarworks.uaeu.ac.ae/cgi/viewcontent.cgi?article=1169&context=all_dissertations |
| رقم الانضمام: | edsbas.41524CB8 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |