Academic Journal
Piezophilic Phenotype Is Growth Condition Dependent and Correlated with the Regulation of Two Sets of ATPase in Deep-Sea Piezophilic Bacterium Photobacterium profundum SS9
| العنوان: | Piezophilic Phenotype Is Growth Condition Dependent and Correlated with the Regulation of Two Sets of ATPase in Deep-Sea Piezophilic Bacterium Photobacterium profundum SS9 |
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| المؤلفون: | Li, An-Qi, Zhang, Wei-Jia, Li, Xue-Gong, Bao, Xu-Chong, Qi, Xiao-Qing, Wu, Long-Fei, Bartlett, Douglas |
| المساهمون: | Laboratoire de chimie bactérienne (LCB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 2076-2607 ; Microorganisms ; https://cnrs.hal.science/hal-04018867 ; Microorganisms, 2023, 11 (3), pp.637. ⟨10.3390/microorganisms11030637⟩. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2023 |
| المجموعة: | Aix-Marseille Université: HAL |
| مصطلحات موضوعية: | deep-sea bacterium, high hydrostatic pressure, piezophilic, ATPase, energy metabolism, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Alteration of respiratory components as a function of pressure is a common strategy developed in deep-sea microorganisms, presumably to adapt to high hydrostatic pressure (HHP). While the electron transport chain and terminal reductases have been extensively studied in deepsea bacteria, little is known about their adaptations for ATP generation. In this study, we showed that the deep-sea bacterium Photobacterium profundum SS9 exhibits a more pronounced piezophilic phenotype when grown in minimal medium supplemented with glucose (MG) than in the routinely used MB2216 complex medium. The intracellular ATP level varied with pressure, but with opposite trends in the two culture media. Between the two ATPase systems encoded in SS9, ATPase-I played a dominant role when cultivated in MB2216, whereas ATPase-II was more abundant in the MG medium, especially at elevated pressure when cells had the lowest ATP level among all conditions tested. Further analyses of the ∆atpI, ∆atpE1 and ∆atpE2 mutants showed that disrupting ATPase-I induced expression of ATPase-II and that the two systems are functionally redundant in MB2216. Collectively, we provide the first examination of the differences and relationships between two ATPase systems in a piezophilic bacterium, and expanded our understanding of the involvement of energy metabolism in pressure adaptation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-04018867; https://cnrs.hal.science/hal-04018867; https://cnrs.hal.science/hal-04018867/document; https://cnrs.hal.science/hal-04018867/file/microorganisms-11-00637.pdf |
| DOI: | 10.3390/microorganisms11030637 |
| الاتاحة: | https://cnrs.hal.science/hal-04018867 https://cnrs.hal.science/hal-04018867/document https://cnrs.hal.science/hal-04018867/file/microorganisms-11-00637.pdf https://doi.org/10.3390/microorganisms11030637 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.4132A770 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/microorganisms11030637 |
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