Academic Journal
Generation and validation of novel adeno-associated viral vectors for the analysis of Ca2+ homeostasis in motor neurons
| العنوان: | Generation and validation of novel adeno-associated viral vectors for the analysis of Ca2+ homeostasis in motor neurons |
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| المؤلفون: | Norante, Rosa Pia, Massimino, Maria Lina, LORENZON, PAOLO, De Mario, Agnese, Peggion, Caterina, VICARIO, MATTIA, Albiero, Mattia, Sorgato, Maria Catia, Lopreiato, Raffaele, Bertoli, Alessandro |
| المساهمون: | Norante, Rosa Pia, Massimino, Maria Lina, Lorenzon, Paolo, De Mario, Agnese, Peggion, Caterina, Vicario, Mattia, Albiero, Mattia, Sorgato, Maria Catia, Lopreiato, Raffaele, Bertoli, Alessandro |
| بيانات النشر: | Nature Publishing Group |
| سنة النشر: | 2017 |
| المجموعة: | Padua Research Archive (IRIS - Università degli Studi di Padova) |
| مصطلحات موضوعية: | Multidisciplinary |
| الوصف: | A finely tuned Ca2+ homeostasis in restricted cell domains is of fundamental importance for neurons, where transient Ca2+ oscillations direct the proper coordination of electro-chemical signals and overall neuronal metabolism. Once such a precise regulation is unbalanced, however, neuronal functions and viability are severely compromised. Accordingly, disturbed Ca2+ metabolism has often been claimed as a major contributor to different neurodegenerative disorders, such as amyotrophic lateral sclerosis that is characterised by selective motor neuron (MN) damage. This notion highlights the need for probes for the specific and precise analysis of local Ca2+ dynamics in MNs. Here, we generated and functionally validated adeno-associated viral vectors for the expression of gene-encoded fluorescent Ca2+ indicators targeted to different cell domains, under the transcriptional control of a MN-specific promoter. We demonstrated that the probes are specifically expressed, and allow reliable local Ca2+ measurements, in MNs from murine primary spinal cord cultures, and can also be expressed in spinal cord MNs in vivo, upon systemic administration to newborn mice. Preliminary analyses using these novel vectors have shown larger cytosolic Ca2+ responses following stimulation of AMPA receptors in the cytosol of primary cultured MNs from a murine genetic model of ALS compared to the healthy counterpart. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/28747684; info:eu-repo/semantics/altIdentifier/wos/WOS:000406364600034; volume:7; issue:1; firstpage:6521; journal:SCIENTIFIC REPORTS; http://hdl.handle.net/11577/3243502; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85026399588; https://www.nature.com/articles/s41598-017-06919-0.pdf |
| DOI: | 10.1038/s41598-017-06919-0 |
| الاتاحة: | http://hdl.handle.net/11577/3243502 https://doi.org/10.1038/s41598-017-06919-0 https://www.nature.com/articles/s41598-017-06919-0.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.412BB25D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-017-06919-0 |
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