A LysM Domain Intervenes in Sequential Protein-Protein and Protein-Peptidoglycan Interactions Important for Spore Coat Assembly in Bacillus subtilis

التفاصيل البيبلوغرافية
العنوان:	A LysM Domain Intervenes in Sequential Protein-Protein and Protein-Peptidoglycan Interactions Important for Spore Coat Assembly in Bacillus subtilis
المؤلفون:	Pereira, Fatima C., Nunes, Filipa, Cruz, Fernando, Fernandes, Catarina, Isidro, Anabela L., Lousa, Diana, Soares, Cláudio M., Moran, Charles P., Henriques, Adriano O., Serrano, Mónica
المساهمون:	Henkin, Tina M., FEDER, HHS \| NIH \| National Institute of General Medical Sciences, MEC \| Fundação para a Ciência e a Tecnologia
المصدر:	Journal of Bacteriology ; volume 201, issue 4 ; ISSN 0021-9193 1098-5530
بيانات النشر:	American Society for Microbiology
سنة النشر:	2019
الوصف:	Bacillus subtilis spores are encased in a multiprotein coat that surrounds an underlying peptidoglycan layer, the cortex. How the connection between the two layers is enforced is not well established. Here, we elucidate the role of the peptidoglycan-binding LysM domain, present in two proteins, SafA and SpoVID, that govern the localization of additional proteins to the coat. We found that SafA LysM is a protein-protein interaction module during the early stages of coat assembly and a cortex-binding module at late stages in morphogenesis, with the cortex-binding function promoting a tight connection between the cortex and the coat. In contrast, SpoVID LysM functions only as a protein-protein interaction domain that targets SpoVID to the spore surface at the onset of coat assembly.
نوع الوثيقة:	article in journal/newspaper
اللغة:	English
DOI:	10.1128/jb.00642-18
DOI:	10.1128/JB.00642-18
الاتاحة:	http://dx.doi.org/10.1128/jb.00642-18 https://journals.asm.org/doi/pdf/10.1128/JB.00642-18
Rights:	https://journals.asm.org/non-commercial-tdm-license
رقم الانضمام:	edsbas.41150571
قاعدة البيانات:	BASE

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الوصف
DOI:	10.1128/jb.00642-18