Academic Journal
Neutrophils dampen adaptive immunity in Brucellosis
| العنوان: | Neutrophils dampen adaptive immunity in Brucellosis |
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| المؤلفون: | Mora-Cartín, Ricardo, Gutiérrez-Jiménez, Cristina, Alfaro-Alarcón, Alejandro, Chaves-Olarte, Esteban, Chacón-Díaz, Carlos, BARQUERO-CALVO, ELIAS, Moreno, Edgardo |
| المصدر: | Infection and Immunity Vol. 87 no. 5 1-14 (2019) |
| بيانات النشر: | American Society For Microbiology |
| سنة النشر: | 2019 |
| المجموعة: | Universidad Nacional Costa Rica (UNA): Repositorio Institucional |
| مصطلحات موضوعية: | BRUCELOSIS, BRUCELLA ABORTUS, NEUTRALIZING ANTIBODIES, NEUTROPHILS, BACTERIAS, ENFERMEDADES INFECCIOSAS, INTERFERON GAMMA, ADAPTIVE IMMUNITY |
| الوصف: | Brucella organisms are intracellular stealth pathogens of animals and humans. The bacteria overcome the assault of innate immunity at early stages of an infection. Removal of polymorphonuclear neutrophils (PMNs) at the onset of adaptive immunity against Brucella abortus favored bacterial elimination in mice. This was associated with higher levels of interferon gamma (IFN-γ) and a higher proportion of cells expressing interleukin 6 (IL-6) and inducible nitric oxide synthase (iNOS), compatible with M1 macrophages, in PMN-depleted B. abortus-infected (PMNd-Br) mice. At later times in the acute infection phase, the amounts of IFN-γ fell while IL-6, IL-10, and IL-12 became the predominant cytokines in PMNd-Br mice. IL-4, IL-1β, and tumor necrosis factor alpha (TNF-α) remained at background levels at all times of the infection. Depletion of PMNs at the acute stages of infection promoted the premature resolution of spleen inflammation. The efficient removal of bacteria in the PMNd-Br mice was not due to an increase of antibodies, since the immunoglobulin isotype responses to Brucella antigens were dampened. Anti-Brucella antibodies abrogated the production of IL-6, IL-10, and IL-12 but did not affect the levels of IFN-γ at later stages of infection in PMNd-Br mice. These results demonstrate that PMNs have an active role in modulating the course of B. abortus infection after the adaptive immune response has already developed. ; Los organismos Brucella son patógenos intracelulares sigilosos de animales y humanos. La bacteria supera el ataque de la inmunidad innata en las primeras etapas de una infección. La eliminación de los neutrófilos polimorfonucleares (PMN) al inicio de la inmunidad adaptativa contra Brucella abortus favoreció la eliminación bacteriana en los ratones. Esto se asoció con niveles más altos de interferón gamma (IFN-γ) y una mayor proporción de células que expresan interleucina 6 (IL-6) y óxido nítrico sintasa inducible (iNOS), compatible con macrófagos M1, en ratones infectados con PMN depleted B. ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://hdl.handle.net/11056/17644 |
| DOI: | 10.1128/IAI.00118-19 |
| الاتاحة: | http://hdl.handle.net/11056/17644 https://doi.org/10.1128/IAI.00118-19 |
| Rights: | Acceso abierto ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.40DE4F69 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/IAI.00118-19 |
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