التفاصيل البيبلوغرافية
| العنوان: |
DataSheet_1_LMP1 and EBNA2 constitute a minimal set of EBV genes for transformation of human B cells.pdf |
| المؤلفون: |
Jingwei Zhang, Thomas Sommermann, Xun Li, Lutz Gieselmann, Kathrin de la Rosa, Maria Stecklum, Florian Klein, Christine Kocks, Klaus Rajewsky |
| سنة النشر: |
2023 |
| مصطلحات موضوعية: |
Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, B cell lymphoma, lymphomagenesis, Epstein-Barr virus (EBV), EBV latent genes, Epstein-Barr nuclear antigen 2 (EBNA2), latent membrane protein 1 (LMP1), lymphoblastoid cell line (LCL), transduction of human primary B cells |
| الوصف: |
Introduction Epstein-Barr virus (EBV) infection in humans is associated with a wide range of diseases including malignancies of different origins, most prominently B cells. Several EBV latent genes are thought to act together in B cell immortalization, but a minimal set of EBV genes sufficient for transformation remains to be identified. Methods Here, we addressed this question by transducing human peripheral B cells from EBV-negative donors with retrovirus expressing the latent EBV genes encoding Latent Membrane Protein (LMP) 1 and 2A and Epstein-Barr Nuclear Antigen (EBNA) 2. Results LMP1 together with EBNA2, but not LMP1 alone or in combination with LMP2A was able to transform human primary B cells. LMP1/EBNA2-immortalized cell lines shared surface markers with EBV-transformed lymphoblastoid cell lines (LCLs). They showed sustained growth for more than 60 days, albeit at a lower growth rate than EBV-transformed LCLs. LMP1/EBNA2-immortalized cell lines generated tumors when transplanted subcutaneously into severely immunodeficient NOG mice. Conclusion Our results identify a minimal set of EBV proteins sufficient for B cell transformation. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/DataSheet_1_LMP1_and_EBNA2_constitute_a_minimal_set_of_EBV_genes_for_transformation_of_human_B_cells_pdf/24863397 |
| DOI: |
10.3389/fimmu.2023.1331730.s001 |
| الاتاحة: |
https://doi.org/10.3389/fimmu.2023.1331730.s001
https://figshare.com/articles/dataset/DataSheet_1_LMP1_and_EBNA2_constitute_a_minimal_set_of_EBV_genes_for_transformation_of_human_B_cells_pdf/24863397 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.40CCCD64 |
| قاعدة البيانات: |
BASE |