Academic Journal
Characterization of soluble microbial products (SMP) derived from glucose and phenol in dual substrate activated sludge bioreactors
| العنوان: | Characterization of soluble microbial products (SMP) derived from glucose and phenol in dual substrate activated sludge bioreactors |
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| المؤلفون: | Magbanua, Benjamin S., Bowers, Alan R. |
| المصدر: | Biotechnology and Bioengineering ; volume 93, issue 5, page 862-870 ; ISSN 0006-3592 1097-0290 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2006 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Soluble microbial products (SMP) generated by activated sludge cultures receiving a mixed feed of phenol and glucose were characterized with respect to molecular weight (MW) distribution, octanol‐water partition coefficient ( K ow ), and Microtox® toxicity. Short‐term batch reactor tests using 14 C‐labeled substrates were performed to collect SMP derived from each substrate, while long‐term tests were performed with SMP accumulated over multiple feed cycles using fed‐batch reactors receiving non‐labeled substrates. Yield of SMP in the batch tests, 10%–20% for phenol and 2%–5% for glucose, differed for each substrate and was independent of initial concentration. The MW distribution (MWD) of SMP was independent of feed composition, and was bimodal in the < 1 kDa and 10–100 kDa MW ranges for phenol‐derived SMP and predominantly < 1 kDa for glucose‐derived SMP. In the non‐labeled tests, the fraction of SMP of MW > 100 kDa increased with the proportion of glucose in the feed. The K ow of phenol‐derived SMP was higher compared to glucose‐derived SMP, indicating that the phenol‐derived SMP were more hydrophobic. This was particularly true at an acidic pH, where the K ow was 4.2 ± 1.0 for phenol‐derived SMP versus 0.13 ± 0.13 for glucose‐derived SMP. Toxicity testing indicated that phenol‐derived SMP, exerting a mean Microtox® inhibition of 1%, were less toxic than phenol itself, and showed little correlation between toxicity and concentration. However, glucose‐derived SMP were generally more toxic than glucose itself (a non‐toxic substrate), and the toxicity increased linearly with the concentration of SMP. © 2006 Wiley Periodicals, Inc. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/bit.20774 |
| الاتاحة: | http://dx.doi.org/10.1002/bit.20774 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.20774 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.20774 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.40AA9B9A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/bit.20774 |
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