Academic Journal
Fatty Acid Supplementation During in vitro Embryo Production Determines Cryosurvival Characteristics of Bovine Blastocysts
| العنوان: | Fatty Acid Supplementation During in vitro Embryo Production Determines Cryosurvival Characteristics of Bovine Blastocysts |
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| المؤلفون: | Aardema, H., Bertijn, I., van Tol, HTA., Rijneveld, A., Vernooij, JCM., Gadella, B. M., Vos, PLAM |
| المصدر: | Frontiers in Cell and Developmental Biology ; volume 10 ; ISSN 2296-634X |
| بيانات النشر: | Frontiers Media SA |
| سنة النشر: | 2022 |
| المجموعة: | Frontiers (Publisher - via CrossRef) |
| الوصف: | In vitro production (IVP) embryos have a reduced quality and poor cryotolerance in comparison to in vivo embryos. This study investigated whether free fatty acid (FFA) conditions, fatty acid free (FAF)- synthetic oviduct fluid (SOF) without or with 25 μM of saturated stearic (C 18:0 ) or unsaturated oleic (C 18:1 ) acid during the first 5 IVP days, relate to quality and cryosurvival of day 8 blastocysts. Apart from the blastocyst scores, both 1) number and size of lipid droplets of fresh blastocysts and 2) total number and apoptotic and necrotic cells, before and after freezing-thawing, were scored by confocal microscopy. Blastocyst rates were significantly lower in the FAF SOF condition in comparison to other groups. Interestingly, blastocysts originating from the C 18:1 group, with a significantly higher lipid content, and blastocysts from the FAF SOF group demonstrated a high cryosurvival rate (70.1 and 67.4%, respectively) comparable with in vivo blastocysts (68%), in contrast to the poor cryosurvival of C 18:0 exposed embryos (17.6%). In all freeze-thawed embryos the average amount of apoptotic and necrotic cells increased albeit that the C 18:0 condition rates were higher (43.2%) when compared to C 18:1 (26.0%) and FAF SOF conditions (26.5%). The current data show that FFA administered during early embryonic development significantly affect the cryotolerance of blastocysts. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.3389/fcell.2022.837405 |
| DOI: | 10.3389/fcell.2022.837405/full |
| الاتاحة: | http://dx.doi.org/10.3389/fcell.2022.837405 https://www.frontiersin.org/articles/10.3389/fcell.2022.837405/full |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.40A865F8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fcell.2022.837405 |
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