Academic Journal
Full-length RNA-Seq of the RHOH gene in human B cells reveals new exons and splicing patterns
| العنوان: | Full-length RNA-Seq of the RHOH gene in human B cells reveals new exons and splicing patterns |
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| المؤلفون: | Leprêtre, Frédéric, Meneboo, Jean-Pascal, Villenet, Celine, Delestré, Laure, Quesnel, Bruno, Shelley, Carl Simon, Figeac, Martin, Galiègue-Zouitina, Sylvie |
| المساهمون: | Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 (PLBS), Institut Pasteur de Lille, Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 (CANTHER), Institut pour la recherche sur le cancer de Lille Lille (IRCL) |
| المصدر: | ISSN: 2045-2322. |
| بيانات النشر: | CCSD Nature Publishing Group |
| سنة النشر: | 2024 |
| المجموعة: | Réseau International des Instituts Pasteur, Paris: HAL-RIIP |
| مصطلحات موضوعية: | New exons, New transcripts, Gene fusion, B-cell differentiation, RHOH gene, Full-length targeted mRNA-Seq, Oxford Nanopore Technologies, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; The RhoH protein is a member of the Ras superfamily of guanosine triphosphate-binding proteins. RhoH is an atypical Rho family member that is always GTP-bound and thus always activated. It is restrictively expressed in normal hematopoietic cells, where it is a negative regulator of cell growth and survival. We previously analyzed the RHOH gene structure and demonstrated that this gene is composed of 7 exons, one single encoding exon located at the 3ʹ extremity of the gene, preceded by 6 noncoding exons. To further understand the transcription events associated with this gene, we performed full-length RNA-Seq on 12 B-cell lines. We identified new exons, new splice events and new splice sites, leading to the discovery of 38 RHOH mRNA molecules, 27 of which have never been described before. Here, we also describe new fusion transcripts. Moreover, our method allowed quantitative measurements of the different mRNA species relative to each other in relation to B-cell differentiation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/39550462; PUBMED: 39550462 |
| DOI: | 10.1038/s41598-024-79307-0 |
| الاتاحة: | https://hal.univ-lille.fr/hal-04836236 https://hal.univ-lille.fr/hal-04836236v1/document https://hal.univ-lille.fr/hal-04836236v1/file/s41598-024-79307-0.pdf https://doi.org/10.1038/s41598-024-79307-0 |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.404D7790 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-024-79307-0 |
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