Academic Journal
Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins.
| العنوان: | Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. |
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| المؤلفون: | Pingzhao Hu, Sarath Chandra Janga, Mohan Babu, J Javier Díaz-Mejía, Gareth Butland, Wenhong Yang, Oxana Pogoutse, Xinghua Guo, Sadhna Phanse, Peter Wong, Shamanta Chandran, Constantine Christopoulos, Anaies Nazarians-Armavil, Negin Karimi Nasseri, Gabriel Musso, Mehrab Ali, Nazila Nazemof, Veronika Eroukova, Ashkan Golshani, Alberto Paccanaro, Jack F Greenblatt, Gabriel Moreno-Hagelsieb, Andrew Emili |
| المصدر: | PLoS Biology, Vol 7, Iss 4, p e96 (2009) |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2009 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Biology (General), QH301-705.5 |
| الوصف: | One-third of the 4,225 protein-coding genes of Escherichia coli K-12 remain functionally unannotated (orphans). Many map to distant clades such as Archaea, suggesting involvement in basic prokaryotic traits, whereas others appear restricted to E. coli, including pathogenic strains. To elucidate the orphans' biological roles, we performed an extensive proteomic survey using affinity-tagged E. coli strains and generated comprehensive genomic context inferences to derive a high-confidence compendium for virtually the entire proteome consisting of 5,993 putative physical interactions and 74,776 putative functional associations, most of which are novel. Clustering of the respective probabilistic networks revealed putative orphan membership in discrete multiprotein complexes and functional modules together with annotated gene products, whereas a machine-learning strategy based on network integration implicated the orphans in specific biological processes. We provide additional experimental evidence supporting orphan participation in protein synthesis, amino acid metabolism, biofilm formation, motility, and assembly of the bacterial cell envelope. This resource provides a "systems-wide" functional blueprint of a model microbe, with insights into the biological and evolutionary significance of previously uncharacterized proteins. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1544-9173 1545-7885 |
| Relation: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19402753/?tool=EBI; https://doaj.org/toc/1544-9173; https://doaj.org/toc/1545-7885; https://doaj.org/article/512d2be209f844b598c2040abfcbe06f |
| DOI: | 10.1371/journal.pbio.1000096 |
| الاتاحة: | https://doi.org/10.1371/journal.pbio.1000096 https://doaj.org/article/512d2be209f844b598c2040abfcbe06f |
| رقم الانضمام: | edsbas.4048B735 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 15449173 15457885 |
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| DOI: | 10.1371/journal.pbio.1000096 |