Academic Journal
Urinary Free Cortisol Determination and Interferences Studies Using Liquid Chromatography Coupled to Tandem Mass Spectrometry after On-Line Solid Phase Extraction Based on TurboflowTM Chromatography
| العنوان: | Urinary Free Cortisol Determination and Interferences Studies Using Liquid Chromatography Coupled to Tandem Mass Spectrometry after On-Line Solid Phase Extraction Based on TurboflowTM Chromatography |
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| المؤلفون: | Bonnet-Serrano, Fidéline, Nakib, Samir, Zientek, Corinne, Guignat, Laurence, Guibourdenche, Jean, Bertherat, Jerôme, Menet, Marie-Claude |
| المساهمون: | Université Paris-Descartes, Sorbonne Paris Cité, 75014 Paris, France, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Specialized Biochemistry Department, Cochin Hospital, 75014 Paris, Hormonology Department, Cochin Hospital, 75014 Paris, France, Reference Center for Rare Adrenal Diseases, Endocrinology Department, Cochin Hospital, 75014 Paris, Hormonology Department, Cochin Hospital, 75014 Paris, Institut de Chimie Physique (ICP), Institut de Chimie - CNRS Chimie (INC-CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay |
| المصدر: | ISSN: 2218-1989 ; Metabolites ; https://hal.science/hal-04292643 ; Metabolites, 2023, 13 (10), pp.1063. ⟨10.3390/metabo13101063⟩. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Cortisol, Extraction en phase solide, Chromatograhy, [CHIM]Chemical Sciences |
| الوصف: | International audience ; (1) A 24 h urinary free cortisol (UFF) is one of the first-line exams recommended for the diagnosis of Cushing’s syndrome. In a hospital hormonology department, this activity can exceed several hundred dosages per week. The UFF is generally determined via an immunoassay with an automate using a chemiluminescence or electrochemiluminescence detection system. To increase the cortisol concentration in the analyzed sample, the automated analysis is preceded by urine extraction, which does not prevent there from being some interferences due to other steroids with close structures. (2) This paper describes the development of on-line solid phase extraction coupled to liquid chromatography and mass spectrometry for the analysis of urinary free cortisol. The on-line extraction was based on the TurboflowTM chromatography coupled to the analytical column by two valves, easily available for the laboratories. (3) The choice of the Accucore Polar Premium® analytical column made it possible to avoid analytical interferences with exogenous or endogenous molecules having the same SRM transition (363 → 121) as cortisol. (4) The method was fully validated in the range of clinically relevant concentrations from the lower limit of quantification (LLOQ) to 411.75 nmol·L−1. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-04292643; https://hal.science/hal-04292643; https://hal.science/hal-04292643v1/document; https://hal.science/hal-04292643v1/file/version%20HAL.pdf |
| DOI: | 10.3390/metabo13101063 |
| الاتاحة: | https://hal.science/hal-04292643 https://hal.science/hal-04292643v1/document https://hal.science/hal-04292643v1/file/version%20HAL.pdf https://doi.org/10.3390/metabo13101063 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.40087448 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/metabo13101063 |
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