Academic Journal
Possibilities of immunocytochemical staining for HBME-1 expression in the «diagnostic search» of differentiated thyroid cancer. Own investigation ; Можливості імуноцитохімічного визначення експресії HBME-1 у діагностичному пошуку диференційованої карциноми щитоподібної залози. Власне дослідження
| العنوان: | Possibilities of immunocytochemical staining for HBME-1 expression in the «diagnostic search» of differentiated thyroid cancer. Own investigation ; Можливості імуноцитохімічного визначення експресії HBME-1 у діагностичному пошуку диференційованої карциноми щитоподібної залози. Власне дослідження |
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| المؤلفون: | Нечай, О. П., Белемець, Н. І., Паламарчук, В. О., Квітка, Д. М., Козачук, Є. С., Товкай, О. А. |
| المصدر: | Clinical Endocrinology and Endocrine Surgery; No. 3 (2023); 5-11 ; Clinical Endocrinology and Endocrine Surgery; № 3 (2023); 5-11 ; 2519-2582 ; 1818-1384 ; 10.30978/10.30978/CEES-2023-3 |
| بيانات النشر: | УНПЦЕХ, ТЕОіТ МОЗ України (URPCES, TEO&T of the Ministry of Health of Ukraine) |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | thyroid cancer, HBME‑1, «Gray zone», рак щитоподібної залози |
| الوصف: | Objective — to investigate HBME-1 expression variants on cytological material classified as doubtful (III, IV, V), according to the Bethesda categories, to evaluate the value of the HBME-1 expression test on the corresponding histological material of nodular goiter, follicular tumor with an uncertain potential for malignancy for the differential diagnosis of thyroid carcinoma (ThC). Materials and methods. The analysis was performed on the data of 14 patients with thyroid nodular lesions and cytological Bethesda categories III, IV, and V (“gray zone“). The HBME-1 expression was determined by the results of immunocytochemical (ICC) and immunohistochemical (IHC) staining with the use of mouse monoclonal antibodies against human HBME-1 (1 : 500; cat № BSB 3459; «Bio SB» «Bioscince for the World» Santa Barbara USA). The HBME-1 expression grade was calculated as the sum of the score for staining intensity and cytoplasmic positive cells square, present in the IHC sample. HBME-1 positive result was considered with an IHC score >6 point. Results. From 14 investigated patients, five were defined as HBME-1 positive. According to the postoperative histological conclusion for the group of HBME-1 positive patients, thyroid carcinoma was diagnosed in four subjects, including three cases of papillary ThC and one follicular ThC; in one patient follicular tumor of uncertain malignant potential (FT-UMP) was reported. Importantly, immunocytochemical and immunohistochemical HBME-1 reactions in the specimens of these patients were of the same intensity. Among 9 HBME-1 preoperative negative patients, none had malignancy according to the final histological study. Nodular goiter was diagnosed in six patients, and tumor of uncertain malignant potential (FT-UMP) was reported for three subjects. Histological specimens of all 9 patients were HBME-1 negative as well. The obtained results demonstrated an increase in cancer detection rates with increasing Bethesda category. Application of statistical software for biomedical research МedCalc ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | Ukrainian |
| Relation: | http://jcees.endocenter.kiev.ua/article/view/288121/281855; http://jcees.endocenter.kiev.ua/article/view/288121 |
| DOI: | 10.30978/CEES-2023-3-5 |
| الاتاحة: | http://jcees.endocenter.kiev.ua/article/view/288121 https://doi.org/10.30978/CEES-2023-3-5 |
| Rights: | Авторське право (c) 2023 Автори ; https://creativecommons.org/licenses/by-nd/4.0/ |
| رقم الانضمام: | edsbas.3FD53A5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.30978/CEES-2023-3-5 |
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