Academic Journal
Against All Odds: Trehalose-6-Phosphate Synthase and Trehalase Genes in the Bdelloid Rotifer Adineta vaga Were Acquired by Horizontal Gene Transfer and Are Upregulated during Desiccation
| العنوان: | Against All Odds: Trehalose-6-Phosphate Synthase and Trehalase Genes in the Bdelloid Rotifer Adineta vaga Were Acquired by Horizontal Gene Transfer and Are Upregulated during Desiccation |
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| المؤلفون: | Hespeels, Boris, Li, Xiang, Flot, Jean-François, Pigneur, Lise-Marie, Malaisse, Jeremy, da Silva, Corinne, van Doninck, Karine |
| المساهمون: | Biologie environnementale et évolutive (URBE), Département de Biologie, Université de Namur Namur (UNamur)-Université de Namur Namur (UNamur), Centre for Biodiversity and Environment Research, Department of Genetics, Evolution and Environment, University College of London London (UCL), Université de Namur Namur (UNamur), Genoscope - Centre national de séquençage Evry (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Namur Center for Complex Systems Namur (NaXys), Belgian National Fund for Scientific Research (Fonds National pour la Recherche Scientifique - FNRS), PhD FRIA (Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture), FRFC (Fonds de la Recherche Fondamentale Collective) grant 2.4.655.09.F, CDR research grant DAMAGE (19597258) |
| المصدر: | ISSN: 1932-6203. |
| بيانات النشر: | HAL CCSD Public Library of Science |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | [SDV.GEN]Life Sciences [q-bio]/Genetics |
| الوصف: | International audience ; The disaccharide sugar trehalose is essential for desiccation resistance in most metazoans that survive dryness; however, neither trehalose nor the enzymes involved in its metabolism have ever been detected in bdelloid rotifers despite their extreme resistance to desiccation. Here we screened the genome of the bdelloid rotifer Adineta vaga for genes involved in trehalose metabolism. We discovered a total of four putative trehalose-6-phosphate synthase (TPS) and seven putative trehalase (TRE) gene copies in the genome of this ameiotic organism; however, no trehalose-6-phosphate phosphatase (TPP) gene or domain was detected. The four TPS copies of A. vaga appear more closely related to plant and fungi proteins, as well as to some protists, whereas the seven TRE copies fall in bacterial clades. Therefore, A. vaga likely acquired its trehalose biosynthesis and hydrolysis genes by horizontal gene transfers. Nearly all residues important for substrate binding in the predicted TPS domains are highly conserved, supporting the hypothesis that several copies of the genes might be functional. Besides, RNAseq library screening showed that trehalase genes were highly expressed compared to TPS genes, explaining probably why trehalose had not been detected in previous studies of bdelloids. A strong overexpression of their TPS genes was observed when bdelloids enter desiccation, suggesting a possible signaling role of trehalose-6-phosphate or trehalose in this process. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | cea-04573313; https://cea.hal.science/cea-04573313; https://cea.hal.science/cea-04573313/document; https://cea.hal.science/cea-04573313/file/file%20%285%29.pdf |
| DOI: | 10.1371/journal.pone.0131313 |
| الاتاحة: | https://cea.hal.science/cea-04573313 https://cea.hal.science/cea-04573313/document https://cea.hal.science/cea-04573313/file/file%20%285%29.pdf https://doi.org/10.1371/journal.pone.0131313 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.3FBEFA7E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0131313 |
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