Academic Journal
Targeting prokineticin system counteracts hypersensitivity, neuroinflammation, and tissue damage in a mouse model of bortezomib-induced peripheral neuropathy
| العنوان: | Targeting prokineticin system counteracts hypersensitivity, neuroinflammation, and tissue damage in a mouse model of bortezomib-induced peripheral neuropathy |
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| المؤلفون: | Moschetti, Giorgia, Amodeo, Giada, Maftei, Daniela, Lattanzi, Roberta, Procacci, Patrizia, Sartori, Patrizia, Balboni, Gianfranco, Onnis, Valentina, Conte, Vincenzo, Panerai, Alberto, Sacerdote, Paola, Franchi, Silvia |
| المساهمون: | Moschetti, Giorgia, Amodeo, Giada, Maftei, Daniela, Lattanzi, Roberta, Procacci, Patrizia, Sartori, Patrizia, Balboni, Gianfranco, Onnis, Valentina, Conte, Vincenzo, Panerai, Alberto, Sacerdote, Paola, Franchi, Silvia |
| سنة النشر: | 2019 |
| المجموعة: | Università degli Studi di Cagliari: UNICA IRIS |
| مصطلحات موضوعية: | Prokineticins, Neuropathic pain, Bortezomib, Neuroinflammation, Macrophages |
| الوصف: | Background: Neuropathy is a dose-limiting side effect of many chemotherapeutics, including bortezomib. The mechanisms underlying this condition are not fully elucidated even if a contribution of neuroinflammation was suggested. Here, we investigated the role of a chemokine family, the prokineticins (PKs), in the development of bortezomib-induced peripheral neuropathy (BIPN), and we used a PK receptor antagonist to counteract the development and progression of the pathology. Methods: Neuropathy was induced in male C57BL/6J mice by using a protocol capable to induce a detectable neuropathic phenotype limiting systemic side effects. The presence of allodynia (both mechanical and thermal) and thermal hyperalgesia was monitored over time. Mice were sacrificed at two different time points: 14 and 28 days after the first bortezomib (BTZ) injection. At these times, PK system activation (PK2 and PK-Rs), macrophage and glial activation markers, and cytokine production were evaluated in the main station involved in pain transmission (sciatic nerve, DRG, and spinal cord), and the effect of a PK receptors antagonist (PC1) on the same behavioral and biochemical parameters was assessed. Structural damage of DRG during BTZ treatment and an eventual protective effect of PC1 were also evaluated. Results: BTZ induces in mice a dose-related allodynia and hyperalgesia and a progressive structural damage to the DRG. We observed a precocious increase of macrophage activation markers and unbalance of pro- and antiinflammatory cytokines in sciatic nerve and DRG together with an upregulation of GFAP in the spinal cord. At higher BTZ cumulative dose PK2 and PK receptors are upregulated in the PNS and in the spinal cord. The therapeutic treatment with the PK-R antagonist PC1 counteracts the development of allodynia and hyperalgesia, ameliorates the structural damage in the PNS, decreases the levels of activated macrophage markers, and prevents full neuroimmune activation in the spinal cord. Conclusions: PK system may be a strategical ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/30995914; info:eu-repo/semantics/altIdentifier/wos/WOS:000465038600004; volume:16; issue:1; numberofpages:16; journal:JOURNAL OF NEUROINFLAMMATION; http://hdl.handle.net/11584/264562; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85064444372; https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-019-1461-0 |
| DOI: | 10.1186/s12974-019-1461-0 |
| الاتاحة: | http://hdl.handle.net/11584/264562 https://doi.org/10.1186/s12974-019-1461-0 https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-019-1461-0 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.3FBE381B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12974-019-1461-0 |
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