Academic Journal
Isolation and Phylogenetic Analysis of a Hunnivirus Strain in Water Buffaloes From China
| العنوان: | Isolation and Phylogenetic Analysis of a Hunnivirus Strain in Water Buffaloes From China |
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| المؤلفون: | Liu, Haifeng, Zhu, Xinyue, Dong, Qinting, Qiao, Chengpeng, Luo, Yuhang, Liu, Yi, Zou, Yanlin, Liu, Huanghao, Wu, Cuilan, Su, Jieyu, Peng, Hao, Ouyang, Kang, Chen, Ying, Li, Jun, Wei, Zuzhang, Huang, Weijian |
| المصدر: | Frontiers in Veterinary Science ; volume 9 ; ISSN 2297-1769 |
| بيانات النشر: | Frontiers Media SA |
| سنة النشر: | 2022 |
| المجموعة: | Frontiers (Publisher - via CrossRef) |
| الوصف: | In recent years, hunniviruses have been reported in a variety of animal species from many countries. Here, hunnivirus was detected in fecal samples from water buffaloes and named as BufHuV-GX-2106. The samples were inoculated into cultures of MDBK cells supplemented with TPCK trypsin and the BufHuV-GX-2106 strain was stably passaged and replicated. Electron microscopic analysis showed the BufHuV-GX-2106 virus particles were spherical and 20~30 nm in diameter. The complete genome of a plaque purified sample of BufHuV-GX-2106 was determined and analyzed. Genomic analysis revealed that the whole sequence of BufHuV-GX-2106 was ~7,601 nucleotides (nt) in length and consisted of a large open reading frame of 6,759nt, a 5′UTR, a 3'UTR and a poly(A) tail. The complete genome sequence of BufHuV-GX-2106 shares 68-85% nucleotide identities with other known hunnivirus strains, indicating high genetic heterogeneity among these viruses. Phylogenetic analysis showed that BufHuV-GX-2106 belonged to the Hunnivirus A species and was more closely related to ovine hunnivirus than other known viruses of this type. This study describes the first isolation and complete genome sequence of a hunnivirus strain from water buffaloes. In addition, this study will help to understand the mechanisms involved in the pathogenesis of Hunnivirus A among different animal species. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.3389/fvets.2022.851743 |
| DOI: | 10.3389/fvets.2022.851743/full |
| الاتاحة: | http://dx.doi.org/10.3389/fvets.2022.851743 https://www.frontiersin.org/articles/10.3389/fvets.2022.851743/full |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.3F28A3D1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fvets.2022.851743 |
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