Academic Journal
Contamination Issue in Viral Metagenomics: Problems, Solutions, and Clinical Perspectives
| العنوان: | Contamination Issue in Viral Metagenomics: Problems, Solutions, and Clinical Perspectives |
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| المؤلفون: | Jurasz, Henryk, Pawłowski, Tomasz, Perlejewski, Karol |
| المساهمون: | Narodowe Centrum Nauki |
| المصدر: | Frontiers in Microbiology ; volume 12 ; ISSN 1664-302X |
| بيانات النشر: | Frontiers Media SA |
| سنة النشر: | 2021 |
| المجموعة: | Frontiers (Publisher - via CrossRef) |
| الوصف: | We describe the most common internal and external sources and types of contamination encountered in viral metagenomic studies and discuss their negative impact on sequencing results, particularly for low-biomass samples and clinical applications. We also propose some basic recommendations for reducing the background noise in viral shotgun metagenomic (SM) studies, which would limit the bias introduced by various classes of contaminants. Regardless of the specific viral SM protocol, contamination cannot be totally avoided; in particular, the issue of reagent contamination should always be addressed with high priority. There is an urgent need for the development and validation of standards for viral metagenomic studies especially if viral SM protocols will be more widely applied in diagnostics. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.3389/fmicb.2021.745076 |
| DOI: | 10.3389/fmicb.2021.745076/full |
| الاتاحة: | http://dx.doi.org/10.3389/fmicb.2021.745076 https://www.frontiersin.org/articles/10.3389/fmicb.2021.745076/full |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.3F1C4CFD |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fmicb.2021.745076 |
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