Academic Journal
Anticancer potentiality of lignan rich fraction of six Flaxseed cultivars
| العنوان: | Anticancer potentiality of lignan rich fraction of six Flaxseed cultivars |
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| المؤلفون: | Ezzat, Shahira M., Shouman, Samia A., Elkhoely, Abeer, Attia, Yasmin M., Elsesy, Mohamed S., El Senousy, Amira S., Choucry, Mouchira A., El Gayed, Sabah H., El Sayed, Abeer A., Sattar, Essam Abdel, El Tanbouly, Nebal |
| المصدر: | Scientific Reports ; volume 8, issue 1 ; ISSN 2045-2322 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2018 |
| الوصف: | The objective of our study is to highlight the therapeutic effect and mechanism of action by which purified Flaxseed hydrolysate (PFH) which is a lignan rich fraction exerts its anticancer activity on a human breast cancer cell line (T47D) and in mice bearing tumor. HPLC analysis of PFH of six flaxseed cultivars had shown that PFH of the cultivar Giza 9 (PFH-G9) contains the highest concentration of SDG (81.64 mg/g). The in vitro cytotoxic potentiality of PFH’s of six flaxseed cultivars was screened against a panel of human cancer cell lines. PFH -G9 showed the most significant cytotoxic activity against ER-receptor positive breast cell lines MCF7 and T47D with IC 50 13.8 and 15.8 µg/ml, respectively. Moreover, PFH-G9 reduced the expression of the metastasis marker, 1-α, metalloproteinases and vascular endothelial growth factor (VEGF), one of the most potent stimulators of angiogenesis, while it increased the caspase-3 dependent apoptosis. Our study also showed that dietary intake of 10% of Giza 9 Flaxseeds (FS), fixed oil (FSO) or Flax meal (FSM) twice daily for 3 weeks in mice-bearing solid Ehrlich ascites carcinoma (EAC) resulted in reducing the tumor volume, the expression of estrogen, insulin growth factor, progesterone, VEGF and MMP-2, but enhanced expression of caspase-3. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41598-017-18944-0 |
| الاتاحة: | http://dx.doi.org/10.1038/s41598-017-18944-0 https://www.nature.com/articles/s41598-017-18944-0.pdf https://www.nature.com/articles/s41598-017-18944-0 |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.3F17E6D6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-017-18944-0 |
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