Academic Journal
Epidemiological Characteristics of Shiga Toxin-Producing Escherichia coli Responsible for Infections in the Polish Pediatric Population
| العنوان: | Epidemiological Characteristics of Shiga Toxin-Producing Escherichia coli Responsible for Infections in the Polish Pediatric Population |
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| المؤلفون: | Seliga-Gąsior Dominika, Sokól-Leszczyñska Beata, Krzysztoñ-Russjan Jolanta, Wierzbicka Diana, Stępieñ-Hołubczat Karolina, Lewandowska Paulina, Frankiewicz Ewa, Cacko Andrzej, Leszczyñska Beata, Demkow Urszula, Podsiadły Edyta |
| المصدر: | Polish Journal of Microbiology, Vol 73, Iss 2, Pp 177-187 (2024) |
| بيانات النشر: | Sciendo |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | shiga toxin-producing escherichia coli, diarrhea, hemolytic uremic syndrome, epidemiology, poland, Genetics, QH426-470, Microbiology, QR1-502 |
| الوصف: | Shiga toxin-producing Escherichia coli (STEC) are zoonotic pathogens causing hemorrhagic colitis and hemolytic uremic syndrome (HUS) in children and the elderly. Stool samples were collected from 180 children hospitalized in five pediatric centers in Poland in 2018–2022. Direct stx1/stx2 gene detection by PCR in feces and E. coli isolates was performed. Antibiotic susceptibility was tested according to EUCAST v.12. Randomly selected isolates were serotyped with O157 antiserum and genotyped by pulsed-field gel electrophoresis (PFGE). A total of 44 E. coli isolates were confirmed as STEC by PCR. Among them, 84.4% were positive for stx2, and equally 6,8% for only stx1 and both stx1 and stx2 genes. The stx1 gene was also found in one Citrobacter freundii isolate. E. coli serotype O157 was present in 97.6% of the isolates. STEC infections most often occurred between June-October with a peak in July and August (51%). The highest, 77.8% of STEC isolates were found in the 1–5 years old group. No extended-spectrum β-lactamases (ESBL) were found. Resistance only to amoxicillin/clavulanic acid (24.4%), piperacillin/tazobactam (3%), cefotaxime (6%), gentamicin (6%), ciprofloxacin (3%), azithromycin (3%), trimethoprim/sulfamethoxazole (24,2%) was detected. PFGE analysis showed 18 PFGE types with no clonal distribution. Eight isolates with A, B, and C PFGE types showed genetic relatedness in the type with no detection of transmission way of distribution. STEC strains pose a serious threat to human health, therefore demographic and epidemiological characteristics are crucial for their surveillance. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2544-4646 |
| Relation: | https://doi.org/10.33073/pjm-2024-016; https://doaj.org/toc/2544-4646; https://doaj.org/article/5570725dec40408d92924fad37f3e9c8 |
| DOI: | 10.33073/pjm-2024-016 |
| الاتاحة: | https://doi.org/10.33073/pjm-2024-016 https://doaj.org/article/5570725dec40408d92924fad37f3e9c8 |
| رقم الانضمام: | edsbas.3E6D57B8 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 25444646 |
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| DOI: | 10.33073/pjm-2024-016 |