Academic Journal
Metabolite and transcript profiling of berry skin during fruit development elucidates differential regulation between Cabernet Sauvignon and Shiraz cultivars at branching points in the polyphenol pathway
| العنوان: | Metabolite and transcript profiling of berry skin during fruit development elucidates differential regulation between Cabernet Sauvignon and Shiraz cultivars at branching points in the polyphenol pathway |
|---|---|
| المؤلفون: | Degu, Asfaw, Hochberg, Uri, Sikron, Noga, Venturini, Luca, Buson, Genny, Ghan, Ryan, Plaschkes, Inbar, Batushansky, Albert, Chalifa Caspi, Vered, Mattivi, Fulvio, Delledonne, Massimo, Pezzotti, Mario, Rachmilevitch, Shimon, Cramer, Grant R., Fait, Aaron |
| المساهمون: | Degu, Asfaw, Hochberg, Uri, Sikron, Noga, Venturini, Luca, Buson, Genny, Ghan, Ryan, Plaschkes, Inbar, Batushansky, Albert, Chalifa Caspi, Vered, Mattivi, Fulvio, Delledonne, Massimo, Pezzotti, Mario, Rachmilevitch, Shimon, Cramer, Grant R., Fait, Aaron |
| سنة النشر: | 2014 |
| المجموعة: | Università degli Studi di Trento: CINECA IRIS |
| مصطلحات موضوعية: | GC-MS, Grape berry metabolism, Grapevine, LC-MS, Metabolite profiling, Metabolomic, Transcript analysi, Plant Science |
| الوصف: | Background: Grapevine berries undergo complex biochemical changes during fruit maturation, many of which are dependent upon the variety and its environment. In order to elucidate the varietal dependent developmental regulation of primary and specialized metabolism, berry skins of Cabernet Sauvignon and Shiraz were subjected to gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–mass spectrometry (LC-MS) based metabolite profiling from pre-veraison to harvest. The generated dataset was augmented with transcript profiling using RNAseq. Results: The analysis of the metabolite data revealed similar developmental patterns of change in primary metabolites between the two cultivars. Nevertheless, towards maturity the extent of change in the major organic acid and sugars (i.e. sucrose, trehalose, malate) and precursors of aromatic and phenolic compounds such as quinate and shikimate was greater in Shiraz compared to Cabernet Sauvignon. In contrast, distinct directional projections on the PCA plot of the two cultivars samples towards maturation when using the specialized metabolite profiles were apparent, suggesting a cultivar-dependent regulation of the specialized metabolism. Generally, Shiraz displayed greater upregulation of the entire polyphenol pathway and specifically higher accumulation of piceid and coumaroyl anthocyanin forms than Cabernet Sauvignon from veraison onwards. Transcript profiling revealed coordinated increased transcript abundance for genes encoding enzymes of committing steps in the phenylpropanoid pathway. The anthocyanin metabolite profile showed F3′5′H-mediated delphinidin-type anthocyanin enrichment in both varieties towards maturation, consistent with the transcript data, indicating that the F3′5′H-governed branching step dominates the anthocyanin profile at late berry development. Correlation analysis confirmed the tightly coordinated metabolic changes during development, and suggested a source-sink relation between the central and specialized metabolism, stronger in ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000339917300001; volume:14; issue:1; journal:BMC PLANT BIOLOGY; http://hdl.handle.net/11572/176850; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84905916813; http://www.biomedcentral.com/1471-2229/14/188 |
| DOI: | 10.1186/s12870-014-0188-4 |
| الاتاحة: | http://hdl.handle.net/11572/176850 https://doi.org/10.1186/s12870-014-0188-4 http://www.biomedcentral.com/1471-2229/14/188 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.3E5CC18B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12870-014-0188-4 |
|---|