Academic Journal
Effects of gadolinium contrast agents in naïve and nephrectomized rats: Relevance to nephrogenic systemic fibrosis
| العنوان: | Effects of gadolinium contrast agents in naïve and nephrectomized rats: Relevance to nephrogenic systemic fibrosis |
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| المؤلفون: | Grant, D., Johnsen, H., Juelsrud, A., Løvhaug, D. |
| المصدر: | Acta Radiologica ; volume 50, issue 2, page 156-169 ; ISSN 0284-1851 1600-0455 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2009 |
| الوصف: | Background: Human nephrogenic systemic fibrosis (NSF) is a rare condition reported in patients with severe renal insufficiency exposed to a gadolinium (Gd)-based contrast agent. An animal model of NSF could help to investigate its mechanisms and lead to prevention and treatment. Purpose: To evaluate a possible animal model of NSF using naïve and partially nephrectomized rats to induce conditions similar to those in patients at risk of NSF. Material and Methods: Naïve rats received intravenous doses of 5 or 10 mmol/kg Omniscan; 5 mmol/kg Magnevist; 1 mmol/kg caldiamide; 1, 2.5, or 5 mmol/kg gadodiamide; 25 µmol/kg GdCl 3 ; or 25 µmol/kg Gd citrate. Partially nephrectomized rats received 5 mmol/kg Omniscan; 5 mmol/kg Magnevist; 1 mmol/kg caldiamide; 1 mmol/kg gadodiamide; 25 µmol/kg GdCl 3 ; or 25 µmol/kg Gd citrate. There were three or four animals per group. Clinical signs were recorded during treatment. At termination, clinical biochemistry, histopathology, and tissue Gd and Zn concentrations were investigated. Results: Similar responses to treatment were seen in naïve and nephrectomized rats. High doses of gadodiamide were toxic, necessitating early termination of the affected animals. Skin lesions appeared in naïve and nephrectomized groups treated with gadodiamide or Omniscan, coinciding with the onset of signs of pruritus, i.e., intensive scratching. The histomorphological features of the skin lesions were also consistent with superficial physical trauma. Dermal fibrosis was not a feature of these skin lesions in any of the groups, i.e., no increased collagen density, CD34+ cells, or increased fibroblasts. This was supported by skinfold measurements that demonstrated no increased skin thickness. Treatment with the gadolinium-based contrast agents and Gd salts resulted in increased Gd content of several tissues. The Gd salts were mainly taken up by the liver and spleen, possibly reflecting formation of insoluble particles and macrophage uptake. Zn tissue concentrations were normal or increased. Other major ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1080/02841850802637808 |
| الاتاحة: | http://dx.doi.org/10.1080/02841850802637808 http://journals.sagepub.com/doi/pdf/10.1080/02841850802637808 |
| Rights: | http://journals.sagepub.com/page/policies/text-and-data-mining-license |
| رقم الانضمام: | edsbas.3E0A984 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1080/02841850802637808 |
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