Academic Journal
Type IV-A3 CRISPR-Cas systems drive inter-plasmid conflicts by acquiring spacers in trans
| العنوان: | Type IV-A3 CRISPR-Cas systems drive inter-plasmid conflicts by acquiring spacers in trans |
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| المؤلفون: | Benz, Fabienne, Camara-Wilpert, Sarah, Russel, Jakob, Wandera, Katharina, Čepaitė, Rimvydė, Ares-Arroyo, Manuel, Gomes-Filho, José Vicente, Englert, Frank, Kuehn, Johannes, Gloor, Silvana, Mestre, Mario Rodríguez, Cuénod, Aline, Aguilà-Sans, Mònica, Maccario, Lorrie, Egli, Adrian, Randau, Lennart, Pausch, Patrick, Rocha, Eduardo, P. C., Beisel, Chase, Madsen, Jonas Stenløkke, Bikard, David, Hall, Alex, Sørensen, Søren Johannes, Pinilla-Redondo, Rafael |
| المساهمون: | Biologie de Synthèse - Synthetic biology, Université Paris Cité (UPCité)-Microbiologie Intégrative et Moléculaire (UMR6047), Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Génomique évolutive des Microbes / Microbial Evolutionary Genomics, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), University of Copenhagen = Københavns Universitet (UCPH), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology Zürich (ETH Zürich), Helmholtz-Institut für RNA-basierte Infektionsforschung - Helmholtz Institute for RNA‐based Infection Research Würzburg, Germany (HIRI), Helmholtz-Zentrum für Infektionsforschung GmbH (HZI)-Julius-Maximilians-Universität Würzburg = University of Würzburg Würsburg, Germany (JMU), Vilnius University Vilnius, Philipps Universität Marburg = Philipps University of Marburg, Université de Bâle = University of Basel = Basel Universität (Unibas), University Hospital Basel Basel, McGill University = Université McGill Montréal, Canada, Universität Zürich Zürich = University of Zurich (UZH), LOEWE Center for Synthetic Microbiology (SYNMIKRO), F.B. was supported by the SNSF grants P1EZP3_195539 and P500PB_210944 . S.C.-W. wassupported by the Lundbeck Foundation grant J.S.M., R250-2017-1392 . M.A.-A wassupported by HORIZON-MSCA-2021-PF-01–01 and EvoPlas-101062386. L.R. was supported byDFG-SPP2141 and the LOEWE Research Cluster Diffusible Signals. J.S.M was supported by theIndependent Research Fund Denmark 0217-00445B . R.P.-R. was supported by a LundbeckFoundation grant R347-2020-2346 . P.P. receives funding from the European RegionalDevelopment Fund under grant agreement number 01.2.2-CPVA-V-716-01-0001 with theCentral Project Management Agency (CPVA), Lithuania, and from the Research Council ofLithuania (LMTLT) under grant agreement number S-MIP-22-10. A.C. was supported by thetwo Cantons of Basel grant PMB-03-17 and by the SNSF grant P500PB_214356 . S.J.S. wasfunded by the Novo Nordisk Foundation PEACE project 0064822. L. M. was supported by theEuropean Union’s Horizon 2020 research and innovation programme under Grant No. 874735(VEO). K.W., F.E. and C.B. were supported by the Deutsche Forschungsgemeinschaft (BE6703/1-2 to C.L.B.)., We thank the Synthetic Biology and Microbial Evolutionary Genomics groups at Institut Pasteur, the Section of Microbiology at the University of Copenhagen, the Phi Lab (Peter Fineran’s group) at Otago University, and the Pathogen Ecology group at ETH Zürich for helpful discussions and mimosas. We thank David Mayo-Muñoz for valuable feedback on the manuscript and Ekaterina Semenova for help with the design of acquisition experiments. We thank Sylvain Brisse (Institut Pasteur) for providing K. pneumoniae strain SB and his group, especially Carla Rodrigues and Chiara Crestani, for help with Oxford Nanopore sequencing. We thank Azimdine Habib, Laure Lemée and Thomas Cokelaer from the Biomics Platform (C2RT, supported by France Génomique ANR-10-INBS-09 and IBISA) at Institut Pasteur for help with RNA sequencing and corresponding analysis in transcriptional repression assays., ANR-10-INBS-0009,France Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), European Project: 101062386,HORIZON-MSCA-2021-PF-01 ,10.3030/101062386,EvoPlas(2022), European Project: 874735,H2020-SC1-2019-Single-Stage-RTD,VEO(2020) |
| المصدر: | ISSN: 1931-3128 ; Cell Host & Microbe ; https://pasteur.hal.science/pasteur-04785210 ; Cell Host & Microbe, 2024, 32 (6), pp.875-886.e9. ⟨10.1016/j.chom.2024.04.016⟩. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | CRISPR-Cas, DinG helicase, Klebsiella pneumoniae, adaptive immunity, antibiotic resistance, inter-plasmid competition, phages, plasmids, type IV CRISPR-Cas, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Plasmid-encoded type IV-A CRISPR-Cas systems lack an acquisition module, feature a DinG helicase instead of a nuclease, and form ribonucleoprotein complexes of unknown biological functions. Type IV-A3 systems are carried by conjugative plasmids that often harbor antibiotic-resistance genes and their CRISPR array contents suggest a role in mediating inter-plasmid conflicts, but this function remains unexplored. Here, we demonstrate that a plasmid-encoded type IV-A3 system co-opts the type I-E adaptation machinery from its host, Klebsiella pneumoniae (K. pneumoniae), to update its CRISPR array. Furthermore, we reveal that robust interference of conjugative plasmids and phages is elicited through CRISPR RNA-dependent transcriptional repression. By silencing plasmid core functions, type IV-A3 impacts the horizontal transfer and stability of targeted plasmids, supporting its role in plasmid competition. Our findings shed light on the mechanisms and ecological function of type IV-A3 systems and demonstrate their practical efficacy for countering antibiotic resistance in clinically relevant strains. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/38754416; info:eu-repo/grantAgreement//101062386/EU/Characterization of the Mobility and Evolution of Non-Transmissible Plasmids/EvoPlas; info:eu-repo/grantAgreement// 874735/EU/Versatile Emerging infectious disease Observatory/VEO; PUBMED: 38754416 |
| DOI: | 10.1016/j.chom.2024.04.016 |
| الاتاحة: | https://pasteur.hal.science/pasteur-04785210 https://pasteur.hal.science/pasteur-04785210v1/document https://pasteur.hal.science/pasteur-04785210v1/file/IVA3_MS_Hal_pasteur.pdf https://doi.org/10.1016/j.chom.2024.04.016 |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/ |
| رقم الانضمام: | edsbas.3DFA657F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.chom.2024.04.016 |
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