التفاصيل البيبلوغرافية
| العنوان: |
Simple Fluorogenic Cellular Assay for Histone Deacetylase Inhibitors Based on Split-Yellow Fluorescent Protein and Intrabodies |
| المؤلفون: |
Yuki Ohmuro-Matsuyama (1844242), Tetsuya Kitaguchi (588172), Hiroshi Kimura (3849), Hiroshi Ueda (52356) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Cell Biology, Genetics, Molecular Biology, Neuroscience, Pharmacology, Biotechnology, Cancer, Virology, Chemical Sciences not elsewhere classified, autism spectrum disorder, H 3K levels, Intrabodies Histone deacetylase, YFP fragment FP 1-10, cell viability test, fragment FP 11, H 3K, split YFP reporter system, HDAC inhibitors, HDAC inhibitor activities, Simple Fluorogenic Cellular Assay, Histone Deacetylase Inhibitors, H 3K intrabody, histone H 3 |
| الوصف: |
Histone deacetylase (HDAC) inhibitors that regulate the posttranslational modifications of histone tails are therapeutic drugs for many diseases such as cancers, neurodegenerative diseases, and asthma; however, convenient and sensitive methods to measure the effect of HDAC inhibitors in cultured mammalian cells remain limited. In this study, a fluorogenic assay was developed to detect the acetylation of lysine 9 on histone H3 (H3K9ac), which is involved in several cancers, Alzheimer’s disease, and autism spectrum disorder. To monitor the changes in H3K9ac levels, an H3K9ac-specific intrabody fused with a small fragment FP11 of the split-yellow fluorescent protein (YFP) (scFv-FP11) was expressed in mammalian cells, together with a larger YFP fragment FP1-10 fused with a nuclear localization signal. When the intranuclear level of H3K9ac is increased, the scFv-FP11 is more enriched in the nucleus via passive diffusion through the nuclear pores from the cytoplasm, which increases the chance of forming a fluorescent complex with the nuclear YFP1-10. The results showed that the YFP fluorescence increased when the cells were treated with HDAC inhibitors. Moreover, the sensitivity of the split YFP reporter system to three HDAC inhibitors was higher than that of a conventional cell viability test. The assay system will be a simple and sensitive detection method to evaluate HDAC inhibitor activities at the levels of both single cells and cell populations. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Simple_Fluorogenic_Cellular_Assay_for_Histone_Deacetylase_Inhibitors_Based_on_Split-Yellow_Fluorescent_Protein_and_Intrabodies/14384435 |
| DOI: |
10.1021/acsomega.0c06281.s001 |
| الاتاحة: |
https://doi.org/10.1021/acsomega.0c06281.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.3D9707FD |
| قاعدة البيانات: |
BASE |