Academic Journal
Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling
| العنوان: | Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling |
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| المؤلفون: | Skabkin, Maxim A., Skabkina, Olga V., Dhote, Vidya, Komar, Anton A., Hellen, Christopher U.T., Pestova, Tatyana V. |
| بيانات النشر: | Cold Spring Harbor Laboratory Press |
| سنة النشر: | 2010 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Research Papers |
| الوصف: | Eukaryotic translation initiation begins with ribosomal recruitment of aminoacylated initiator tRNA (Met-tRNAMet i ) by eukaryotic initiation factor eIF2. In cooperation with eIF3, eIF1, and eIF1A, Met-tRNAMet i /eIF2/GTP binds to 40S subunits yielding 43S preinitiation complexes that attach to the 5′-terminal region of mRNAs and then scan to the initiation codon to form 48S initiation complexes with established codon–anticodon base-pairing. Stress-activated phosphorylation of eIF2α reduces the level of active eIF2, globally inhibiting translation. However, translation of several viral mRNAs, including Sindbis virus (SV) 26S mRNA and mRNAs containing hepatitis C virus (HCV)-like IRESs, is wholly or partially resistant to inhibition by eIF2 phosphorylation, despite requiring Met-tRNAMet i . Here we report the identification of related proteins that individually (Ligatin) or together (the oncogene MCT-1 and DENR, which are homologous to N-terminal and C-terminal regions of Ligatin, respectively) promote efficient eIF2-independent recruitment of Met-tRNAMet i to 40S/mRNA complexes, if attachment of 40S subunits to the mRNA places the initiation codon directly in the P site, as on HCV-like IRESs and, as we show here, SV 26S mRNA. In addition to their role in initiation, Ligatin and MCT-1/DENR can promote release of deacylated tRNA and mRNA from recycled 40S subunits after ABCE1-mediated dissociation of post-termination ribosomes. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://genesdev.cshlp.org/cgi/content/short/24/16/1787; http://dx.doi.org/10.1101/gad.1957510 |
| DOI: | 10.1101/gad.1957510 |
| الاتاحة: | http://genesdev.cshlp.org/cgi/content/short/24/16/1787 https://doi.org/10.1101/gad.1957510 |
| Rights: | Copyright (C) 2010, Cold Spring Harbor Laboratory Press |
| رقم الانضمام: | edsbas.3D823D1D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/gad.1957510 |
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