Academic Journal
Large-scale international validation of an indirect ELISA based on recombinant nucleocapsid protein of rift valley fever virus for the detection of IgG antibody in domestic ruminants
| العنوان: | Large-scale international validation of an indirect ELISA based on recombinant nucleocapsid protein of rift valley fever virus for the detection of IgG antibody in domestic ruminants |
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| المؤلفون: | Paweska, Janusz Tadeusz, Jansen van Vuren, Petrus, Msimang, Veerle, Lo, Modu Moustapha, Thiongane, Yaya, Mulumba-Mfumu, Leopold K., Mansoor, Alqadasi, Fafetine, Jose M., Magona, Joseph W., Boussini, Hiver, Bazanow, Barbara, Wilson, William C., Pepin, Michel, Unger, H., Viljoen, Gerrit |
| بيانات النشر: | MDPI |
| سنة النشر: | 2021 |
| المجموعة: | University of Pretoria: UPSpace |
| مصطلحات موضوعية: | Recombinant nucleocapsid, IgG antibody, Domestic ruminants, Validation, Diagnostic accuracy, Rift Valley fever virus (RVFV), Indirect enzyme-linked immunosorbent assay (I-ELISA) |
| الوصف: | Diagnostic performance of an indirect enzyme-linked immunosorbent assay (I-ELISA) based on a recombinant nucleocapsid protein (rNP) of the Rift Valley fever virus (RVFV) was validated for the detection of the IgG antibody in sheep (n = 3367), goat (n = 2632), and cattle (n = 3819) sera. Validation data sets were dichotomized according to the results of a virus neutralization test in sera obtained from RVF-endemic (Burkina Faso, Democratic Republic of Congo, Mozambique, Senegal, Uganda, and Yemen) and RVF-free countries (France, Poland, and the USA). Cut-off values were defined using the two-graph receiver operating characteristic analysis. Estimates of the diagnostic specificity of the RVFV rNP I-ELISA in animals from RVF-endemic countries ranged from 98.6% (cattle) to 99.5% (sheep) while in those originating from RVF-free countries, they ranged from 97.7% (sheep) to 98.1% (goats). Estimates of the diagnostic sensitivity in ruminants from RVF-endemic countries ranged from 90.7% (cattle) to 100% (goats). The results of this large-scale international validation study demonstrate the high diagnostic accuracy of the RVFV rNP I-ELISA. Standard incubation and inactivation procedures evaluated did not have an adverse effect on the detectable levels of the anti-RVFV IgG in ruminant sera and thus, together with recombinant antigen-based I-ELISA, provide a simple, safe, and robust diagnostic platform that can be automated and carried out outside expensive bio-containment facilities. These advantages are particularly important for less-resourced countries where there is a need to accelerate and improve RVF surveillance and research on epidemiology as well as to advance disease control measures. ; The International Atomic Energy Agency ; http://www.mdpi.com/journal/viruses ; pm2021 ; Medical Virology |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | 1999-4915 (online); https://repository.up.ac.za/handle/2263/84858 |
| DOI: | 10.3390/v13081651 |
| الاتاحة: | https://doi.org/10.3390/v13081651 https://repository.up.ac.za/handle/2263/84858 |
| Rights: | © 2021 by the authors. Licensee: MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. |
| رقم الانضمام: | edsbas.3D3FB21F |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/v13081651 |
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