Academic Journal
Epitranscriptomic profiling of N4-acetylcytidine-related RNA acetylation in the spinal dorsal horn of rat with cancer-induced bone pain
| العنوان: | Epitranscriptomic profiling of N4-acetylcytidine-related RNA acetylation in the spinal dorsal horn of rat with cancer-induced bone pain |
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| المؤلفون: | Xu, Longsheng, Zheng, Shang, Liu, Beibei, Xu, Chengfei, Yang, Lei, Zhou, Qinghe, Yao, Ming, Li, Xiang-yao |
| المساهمون: | Zhejiang Multidisciplinary Innovation Team of Traditional Chinese Medicine for Diagnosis and Treatment of Elderly Headache and Vertigo, Medical and Health Research Project of Zhejiang Province, National Natural Science Foundation of China, Natural Science Foundation of Zhejiang Province |
| المصدر: | Molecular Pain ; volume 19 ; ISSN 1744-8069 1744-8069 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2023 |
| الوصف: | Recently, epigenetics involved in the regulation of gene expression has become a research hotspot. This study evaluated N4-acetylcytidine (ac4c) RNA acetylation in the spinal dorsal horn (SDH) of rats with cancer-induced bone pain (CIBP). The ac4C-specific RIP sequencing and NAT10-specific RIP sequencing were performed to identify the differences in ac4C acetylation and gene expression in the SDH between CIBP and sham groups, the relationship with the acetylation-modifying enzyme NAT10, and association analysis was performed. By interfering with the NAT10 expression, the relationship between some up-regulated genes and ac4C acetylation in CIBP was verified. In this study, we demonstrated that bone cancer increases the levels of NAT10 and the overall acetylation, inducing differential ac4C patterns in the SDH of rats. Through verification experiments, it was found that ac4C acetylation of some genes is regulated by NAT10, and differential ac4C patterns in RNA determine the expression of this RNA. We exposed that some CIBP-related gene expression was altered in the SDH of rats, which was regulated by differentially expressed ac4C acetylation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/17448069231178487 |
| الاتاحة: | https://doi.org/10.1177/17448069231178487 https://journals.sagepub.com/doi/pdf/10.1177/17448069231178487 https://journals.sagepub.com/doi/full-xml/10.1177/17448069231178487 |
| Rights: | https://creativecommons.org/licenses/by-nc/4.0/ |
| رقم الانضمام: | edsbas.3D2C202F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/17448069231178487 |
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