Academic Journal
Phylogenetic diversity and virulence gene characteristics of Escherichia coli from pork and patients with urinary tract infections in Thailand
| العنوان: | Phylogenetic diversity and virulence gene characteristics of Escherichia coli from pork and patients with urinary tract infections in Thailand |
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| المؤلفون: | Ketkhao, Pramualchai, Utrarachkij, Fuangfa, Parikumsil, Nattaya, Poonchareon, Kritchai, Kerdsin, Anusak, Ekchariyawat, Peeraya, Narongpun, Pawarut, Nakajima, Chie, Suzuki, Yasuhiko, Suthienkul, Orasa |
| المساهمون: | Asare, Kwame Kumi, Japan Agency for Medical Research and Development, Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan |
| المصدر: | PLOS ONE ; volume 19, issue 7, page e0307544 ; ISSN 1932-6203 |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2024 |
| المجموعة: | PLOS Publications (via CrossRef) |
| الوصف: | Extraintestinal pathogenic Escherichia coli (ExPEC), especially uropathogenic E . coli (UPEC) are responsible for urinary tract infections (UTIs), while diarrheagenic E . coli (DEC) cause foodborne illnesses. These pathogenic E . coli are a serious threat to human health and a public concern worldwide. However, the evidence on pork E . coli (PEC) harboring UPEC virulence-associated genes is currently limited. Therefore, this study aimed to determine the phylogroups, virulence genes, and their association between PEC and UPEC from UTI patients. In this study, 330 E . coli were obtained from archived stock culture isolated from pork (PEC; n = 165) and urine of patients with UTIs (UPEC; n = 165) during 2014–2022. Phylogroups, UPEC- and diarrheagenic E . coli (DEC) associated virulence genes were assessed using PCR assays. The results showed that phylogroups A (50.3%), and B1 (32.1%) were commonly found among PEC whereas phylogroups B2 (41.8%), and C (25.5%) were commonly detected in the UPEC. PEC and UPEC carried similar virulence-associated genes with different percentages. The most frequent UPEC virulence-associated gene among UPEC, and PEC strains was fimH , (93.3%, and 92.1%), followed by iucC (55.2%, and 12.7%), papC (21.8%, and 4.2%), afaC (22.4%, and 0%), hlyCA (17%, and 0.6%), cnf (16.4%, and 0.6%), and sfa/focDE (8.5%, and 4.8%). Additionally, 6 of 27 UPEC virulence-associated gene patterns were found in both PEC and UPEC strains regardless of phylogroups. Furthermore, the DEC virulence-associated genes were found in only 3 strains, one from PEC harboring eae , and two from UPEC carried fimH - bfpA or afaC - CVD432 indicating hybrid strains. Cluster analysis showed a relationship between PEC and UPEC strains and demonstrated that PEC harboring UPEC virulence-associated genes in pork may be associated with UPEC in humans. Food safety and hygiene practices during pork production chain are important procedures for minimizing cross-contamination of these strains that could be transmitted to the consumers. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1371/journal.pone.0307544 |
| الاتاحة: | http://dx.doi.org/10.1371/journal.pone.0307544 https://dx.plos.org/10.1371/journal.pone.0307544 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.3C919EC5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0307544 |
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