Academic Journal
Induction of macrophage colony-stimulating factor-dependent growth and differentiation after introduction of the murine c-fms gene into FDC-P1 cells.
| العنوان: | Induction of macrophage colony-stimulating factor-dependent growth and differentiation after introduction of the murine c-fms gene into FDC-P1 cells. |
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| المؤلفون: | Rohrschneider, L R, Metcalf, D |
| المصدر: | Molecular and Cellular Biology ; volume 9, issue 11, page 5081-5092 ; ISSN 0270-7306 1098-5549 |
| بيانات النشر: | Informa UK Limited |
| سنة النشر: | 1989 |
| الوصف: | A system has been established for analyzing the functions of the c-fms/macrophage colony-stimulating factor (M-CSF) receptor gene product in hematopoietic growth and differentiation. The murine c-fms gene was introduced into the factor-dependent murine hematopoietic cell line FDC-P1 by retroviral infection, and conversion to M-CSF-dependent growth was assayed in agar cultures. Expression of the c-fms gene in FDC-P1 cells, which normally do not express this gene, resulted in the conversion of resultant FD(c-fms) cells to M-CSF-dependent growth. Stimulation of FD(c-fms) cells by M-CSF led to the formation of colonies of altered morphology and produced reversible morphological changes suggestive of myeloid differentiation. M-CSF also induced expression of mature myeloid surface marker proteins in the FD(c-fms) cells. Neither multi-CSF nor granulocyte-macrophage CSF induced similar phenotypic changes but remained able to stimulate the proliferation of undifferentiated FD(c-fms) cells. These results indicate that the c-fms gene was expressed functionally in FDC-P1 cells and transmitted signals for growth. Also, the interaction of M-CSF with the c-fms gene product generated an additional signal for myeloid differentiation but did not irreversibly commit FD(c-fms) cells to terminal differentiation. This system can be used for molecular analysis of the growth- and differentiation-promoting activities of the c-fms proto-oncogene. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/mcb.9.11.5081 |
| DOI: | 10.1128/MCB.9.11.5081 |
| الاتاحة: | http://dx.doi.org/10.1128/mcb.9.11.5081 https://syndication.highwire.org/content/doi/10.1128/MCB.9.11.5081 |
| رقم الانضمام: | edsbas.3C889FAF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/mcb.9.11.5081 |
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